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NAD + -aminoaldehyde dehydrogenase candidates for 4-aminobutyrate (GABA) and β-alanine production during terminal oxidation of polyamines in service apple fruit. Sexuality In Beowulf:? E-mail address: christophert@nutriag.com Department of Plant Agriculture, University of Guelph, Guelph, Ontario N1G 2W1, Canada Search for more papers by this author 1 Present address: NutriAg Ltd., 62 Arrow Rd, North York, Ontario M9M 2L8, Canada. Corresponding author. Fax: +1 519 767 0755. First published: 18 August 2015 Full publication history DOI: 10.1016/j.febslet.2015.08.005 View/save citation Cited by (CrossRef): 0 articles Check for updates. Writing Service? The last step of polyamine catabolism involves the oxidation of 3-aminopropanal or 4-aminobutanal via aminoaldehyde dehydrogenase. In this study, two apple ( Malus x domestica ) AMADH genes were selected ( MdAMADH1 and MdAMADH2 ) as candidates for encoding 4-aminobutanal dehydrogenase activity.

Maximal activity and catalytic efficiency were obtained with NAD + and 3-aminopropanal, followed by 4-aminobutanal, at Soliloquy Essay pH 9.8. NAD + reduction was accompanied by the production of GABA and β-alanine, respectively, when 4-aminobutanal and 3-aminopropanal were utilized as substrates. Md AMADH2 was peroxisomal and service, Md AMADH1 cytosolic. These findings shed light on the potential role of apple AMADHs in 4-aminobutyrate and β-alanine production. 4-Aminobutyrate (GABA) is a ubiquitous, non-proteinaceous four-carbon amino acid that functions in metabolism and cell signaling. GABA markedly accumulates in Essay on The Sexuality Of Women In Beowulf: Film vs. Print plants subjected to writing, various biotic and abiotic stresses including anoxia, chilling, drought, salinity and mechanical damage[13] and is biosynthesized via two distinct pathways. The Benefits Of Hot? GABA can be derived from glutamate via pH- and calmodulin-dependent glutamate decarboxylase activity in the first step of the GABA-shunt pathway[4,5]. Alternatively, oxidation of putrescine and spermidine can contribute to thesis writing, GABA production[6]. In dicotyledonous plants, the copper-containing amine oxidases (CuAO; EC 1.4.3.22) are responsible for the conversion of putrescine to 4-aminobutanal (ABAL), spermidine to 1,3-diaminopropane and ABAL, and 1,3-diaminopropane to 3-aminopropanal (APAL)[7,8]. ABAL is in rapid non-enzymatic equilibrium with Δ −1 -pyrroline, and the oxidation of these two metabolites is often considered to be catalyzed by the same enzyme, generally referred to as NAD + -dependent aminoaldehyde dehydrogenase (AMADH, EC 1.2.1.19)[6], leading to the biosynthesis of GABA.

The activity of plant AMADHs has generally been determined as substrate-dependent NADH production and there is only preliminary evidence, using excised tissue or crude protein extract, for the production of GABA from ABAL[9,10]. Creole Man? AMADHs are classified into thesis, several different aldehyde dehydrogenase families (ALDH9, ALDH10, ALDH25, ALDH26 and ALDH27)[11,12]. ALDH9 enzymes are found in creole man animals, fungi and bacteria, whereas ALDH10 enzymes are found in plants and bacteria, and ALDH25, ALDH26 and ALDH27 enzymes are found in bacteria. All plant AMADHs classified as ALDH10 display 7085% sequence identity to thesis writing service, each other and War Started? Essay, 3539% sequence identity with bacterial, fungal or mammal orthologs from the other families[1315]. Writing? Genome analysis of several higher plant species has revealed two homologous AMADH genes per species, with the exception of cucumber, grape and maize[1416]. Historically, the AMADHs have been frequently designated as 4-aminoaldehyde dehydrogenase, 4-guanidinobutyraldehyde dehydrogenase, betaine aldehyde (BAL) dehydrogenase or trimethylaminobutyraldehyde dehydrogenase, depending on substrate preference. Missihoun et al.[16] reported the existence of two putative AMADH genes in Arabidopsis , AtALDH10A8 and AtALDH10A9 , although it was possible to generate and purify recombinant At ALDH10A9 only in on The Of Women In Beowulf: sufficient quantities for demonstration of NAD + reduction in the presence of writing, BAL, ABAL or APAL. Creole Man? Furthermore, these authors suggested that At ALDH10A8 and At ALDH10A9 are targeted to thesis service, leucoplast and peroxisome, respectively. Study of the biochemical properties of plant AMADHs has become attractive because of the potential involvement of these enzymes in important physiological processes.

For example, Basmati or Jasmine rice, which lacks a functional ABAL dehydrogenase protein, acetylate ABAL (or its cyclic form Δ 1 -pyrroline), resulting in the accumulation of 2-acetyl-Δ 1 -pyrroline, a potent component of rice fragrance[17]. Water? Plant AMADHs also catalyze the biosynthesis of thesis writing service, osmoprotectants such as glycine betaine via the oxidation of BAL in the chloroplast stroma[14,18]. Other research has described the accumulation of GABA in fruits, particularly apple, stored under controlled atmosphere conditions, which include low temperature, low O 2 and elevated CO 2 (03 C, 12.5 kPa O 2 , 220 kPa CO 2 )[6,19,20]. Of Hot Water? Previous research has demonstrated that the peroxisomal MdAO1 gene is highly expressed in apple fruit and the encoded protein prefers diamines such 1,3-diaminopropane, putrescine and cadaverine as substrates[7]. In the thesis writing present study, two AMADH genes were identified and cloned from the ALDH10 gene family (designated as MdAMADH1 and MdAMADH2 ). These two genes were heterologously expressed and the resultant recombinant proteins were purified and biochemically characterized. Both proteins catalyzed the Why The Civil War Started? NAD + -dependent oxidation of APAL and ABAL to β-alanine and GABA, respectively, but BAL was a very poor substrate.

Transient expression of writing service, green fluorescent protein (GFP) fusions in Arabidopsis protoplasts revealed that Md AMADH2 is peroxisomal, whereas Md AMADH1 is cytosolic. Details of the cloning strategy and Why The Essay, primer list are provided in theSupplementary Materials and thesis service, Methods. 2.2 MdAMADH expression in various plant organs. Total RNA was extracted from the frozen powder of creole man, mature fruit, fully expanded leaves, and opened flowers as described previously[7]. Thesis Service? One microgram of Dnase I (Ambion) treated total RNA was used for first-strand cDNA synthesis with Oligo( dT ) 20 and Superscript III (Invitrogen, Carlsbad, CA) at 50 C for 30 min followed by 55 C, according to the manufacturer's protocol. Quantitative real time PCR was conducted as described previously[7]. Relative expression and data analysis were determined using the 2 −ΔCt method[21] and the apple Elongation factor 1-α ( MdEF-1α , Apple Genome Database MDP0000294265), as a superior housekeeping gene that is constitutively expressed[22,23]. The efficiency of the primer pairs was determined and only pairs with 90105% efficiency were selected for further experiments. Two technical replicates were averaged for each biological replicate, and the mean of Essay about With a Human Manger, three biological replicates was determined for each organ. Primer sequences utilized in gene expression of MdAMADH1 , MdAMADH2 and MdEF-1α are listed inSupplementary Table S1.

2.3 Expression and purification of thesis writing service, recombinant proteins. Escherichia coli strain BL21 cells (EMD Millipore) were transformed with standard transformation methods[24]. Recombinant protein expression was induced by adding 0.4 mM isopropyl β- d -1-thiogalactopyranoside to the Lysogeny broth medium supplemented with 50 μg mL −1 ampicillin after attaining an OD 600 of 0.5. The cells were harvested 4 h after induction and then stored at creole man −80 C. Bacterial lysis and writing service, purification of recombinant protein were performed as described elsewhere[7]. Fractions containing high protein levels were pooled and precipitated on ice by slowly adding ammonium sulfate, with gentle stirring, to 80% saturation.

Total proteins were visualized by undergraduate and postgraduate, SDSPAGE gel electrophoresis, followed by thesis writing service, staining with Coomassie Blue R-250 using standard protocols. On The Of Women Film Vs. Print? Immunoblot analysis was based on thesis writing service, a semi-dry method using a mouse monoclonal IgG against Soliloquy Analysis the His probe (Santa Cruz Biotechnology, 1:1000) and an anti-mouse IgGAlkaline Phosphatase (Sigma, 1:10,000) as primary and secondary antibodies, respectively[7]. The Alkaline Phosphatase Conjugate Substrate Kit (Bio-Rad) was used to visualize fusion proteins. Enzymatic activity was assayed as the substrate-dependent production of NADH[13] or GABA and β-alanine[25]. Details are provided in theSupplemental Materials and Methods. Thesis Service? 2.5 Transient transformation of Arabidopsis cell suspension protoplasts.

Protoplasts were prepared from Arabidopsis thaliana cell suspension culture ecotype Col-0 as described previously[26]. N- and C-terminal GFP-fusion plasmids containing apple AMADHs were co-transformed with either mCherry peroxisome (pRTL2/Cherry-PTS1)[27], or cytosolic red fluorescent protein (RFP) (pRTL2-MCS-RFP-stop)[28] marker. Protoplasts were transformed using polyethylene glycol 4000 (Sigma) as described previously[29,30]. About Interview A Human Resources Manger? Confocal laser scanning microscopy was performed with an upright Leica DM 6000B microscope connected to a Leica TCS SP5 system. The excitation/emission scan settings were 488 nm/505 nm for the mGFP channel and 543 nm/610 nm for writing service the RFP/mcherry channel. Modulation of laser light intensity and time-lapse scanning was performed using the Leica software (LAS AF). 3.1 Sequence comparison, conserved motifs and Water, phylogenetic relationship of MdAMADHs and some characterized AMADHs.

In silico analysis of the apple genome database revealed two putative members of the ALDH10 gene family. Full length genes were cloned using apple fruit cDNA, followed by rapid amplification of cDNA ends. These genes were designated by the common names MdAMADH1 (GenBank Acc. No. KP218040) and MdAMADH2 (GenBank Acc. Thesis? No. KP218041) for our purposes, but were recently classified by the ALDH nomenclature committee[11] as ALDH10A9 and creole man, ALDH10A8, respectively. MdAMADH1 and MdAMADH2 encode proteins of 55.0 and 54.6 kDa, respectively, and are 94.8% identical to each other. Md AMADH1 shares 82.6% identity with A. Thesis? thaliana At ALDH10A8 and 79.1% identity with At ALDH10A9, whereas Md AMADH2 shares 82.8% identity with At ALDH10A8 and 80.5% identity with At ALDH10A9.

Comparison of the of Hot putative AMADHs from apple with characterized AMADHs from other plant species reveals that apple and Arabidopsis AMADH isoforms are very similar to thesis writing service, each other, suggesting that the two members of the ALDH10 family in Water Heaters Essay various species are the result of independent gene duplication[15]. Sequence comparisons indicate that apple AMADHs possess three highly conserved catalytic residues (N162, E260 and C294, Md AMADH numbering), which form the writing active site in Ps AMADHs[31], and two conserved aspartate residues located at the entrance of the substrate channel (D110, D113), as well as Y163 and W288, which are considered essential for Essay Interview a Human Manger high-affinity binding of ω-aminoaldehydes such as APAL[32] (Supplementary Fig. S1). Together, these data suggest that Md AMADHs are typical AMADHs in the ALDH10 family. 3.2 Expression analysis of MdAMADHs.

The levels of MdAMADH1 or MdAMADH2 expression were similar across fruit, leaves and flowers (Fig. 1 ). However, the expression of MdAMADH1 was two to three times that for MdAMADH2 in all organs studied. Organ-specific expression of two MdAMADH genes. Transcript abundance in corresponding organs was quantified by thesis writing, real-time RT-PCR using gene-specific primers and normalized to a housekeeping gene, EF-1α . Data represent the mean ± S.E. of three biological replicates. 3.3 Biochemical properties and substrate specificity of apple AMADHs. Difference Uk? The cDNAs for MdAMADH1 , MdAMADH2 were individually expressed in E. coli and the recombinant His-tagged proteins purified to homogeneity, as indicated by Coomassie blue staining and writing, immunoblot analysis of the soluble and affinity-purified fractions (Supplementary Fig. S2).

Enzyme activity was determined as the creole man production of writing, NADH during the With Manger oxidation of thesis, APAL, ABAL or BAL; activity with BAL was detected only after increasing the protein concentration in the enzymatic reaction to ten times the Why The War Started? level used for the APAL-dependent reaction (i.e., 100 nM). Initial measurements of maximal BAL-dependent activity were two to three orders of magnitude less than those for APAL and therefore BAL was omitted from the kinetic studies. Stability tests revealed that recombinant apple AMADH proteins retained their enzymatic activity for service several months after ammonium sulfate precipitation and storage at −80 C. The activities of Md AMADH1and Md AMADH2 displayed a sharp pH optimum at pH 9.8, a similar pattern over the pH range 711, and 20% of their maximal activity at pH 8 (Fig. 2 ). The maximal APAL-dependent activities of Md AMADH1 and Md MADH2 with saturating NADP + were only 1020% of Civil Essay, those with saturating NAD + (data not shown). Thesis Writing Service? Activity of creole man, Md AMADHs as a function of pH. Writing? Assays were run at room temperature with HEPESGlycine buffer, 1 mM ABAL and 1 mM NAD + . Activity is expressed as a percent of the The Benefits of Hot activity at the pH optimum (100%: 1.2 and 1.5 μmol −1 min −1 mg −1 for thesis writing Md AMADH1 and Md AMADH2, respectively).

Data represent the mean of four technical replicates from a typical enzyme preparation. NAD + -dependent activities at creole man pH 9.8 demonstrated that Md AMADH1 and Md AMADH2 display partial substrate inhibition for both APAL and ABAL (Table 1 ;Supplementary Fig. S3). Thesis Writing Service? The enzymes have approximately four to 18 times higher affinity and Civil War Started?, 65120 times higher catalytic efficiency for APAL than ABAL. APAL displays ten to 50 times stronger inhibition than ABAL across the two enzymes.

These findings are in thesis service general agreement with previous reports for Zm AMADH1a, Zm AMADH1b, Zm AMADH2, Ps AMADH1 and Ps AMADH2[13,31]. When APAL was provided at Iago's Analysis a subsaturating level to limit substrate inhibition and allow for sufficient catalytic rates, there was a three-fold range in affinity for NAD + across the enzymes (Table 1). The kinetic parameters for thesis each Md AMADH were determined using a typical enzyme preparation and a reaction solution containing 50 mM glycineNaOH buffer at Heaters pH 9.8, 1 mM NAD + , 10% glycerol, 10 mM 2-mercaptoethanol and various concentrations of ABAL or APAL. The data were fit to the appropriate MichaelisMenten equation using non-linear regression. The kinetic curves are shown inSupplementary Fig. S3.

V max (μmol min −1 mg −1 protein), K m (μM), catalytic efficiency ( k cat / K m ) and substrate inhibition constant ( K is , μM) are shown for service each substrate or coenzyme, as appropriate. NAD + dependence was estimated in the presence of creole man, 40 μM APAL to minimize substrate inhibition; therefore, these parameters are apparent values. Writing Service? To confirm the biochemical function of the Md AMADHs, ABAL or APAL was supplied to creole man, the recombinant proteins in vitro at concentrations approximately two times their corresponding K m values in order to minimize substrate inhibition. The production of GABA or β-alanine was readily detectable within 30 s after initiating the writing reactions, and the accumulation of these products over Essay about Interview a Human Resources, time depended on thesis writing service, enzyme concentration and substrate preference (Fig. 3 ). Notably, the estimated initial reaction rates were similar to those obtained by The Benefits Heaters Essay, monitoring NADH production (Supplementary Fig. S3). Furthermore, the accumulation of β-alanine rapidly reached a plateau, even though 5669% of the substrate remained, suggesting that enzymatic activity was subject to product inhibition (Fig. 3).

Production of GABA and β-alanine by recombinant Md AMADH1 (A) and Md AMADH2 (B) incubated with ABAL or APAL. For assay of ABAL-dependent activities, the 5 mL reactions contained 50 nM protein, 1 mM NAD + and thesis writing service, 200 ( Md AMADH1) or 300 ( Md AMADH2) μM ABAL, whereas for assay of APAL-dependent activities, the Soliloquy reactions contained 10 nM protein, 1 mM NAD + and 30 ( Md AMADH1) or 20 ( Md AMADH2) μM APAL. Data represent the mean of three technical replicates from a typical enzyme preparation. Closed and open circles represent GABA and β-alanine, respectively. The estimated rates of GABA production at 30 s were 0.96 and 2.16 μmol min −1 mg −1 protein for Md AMADH1 and thesis writing, Md AMADH2, respectively. The corresponding rates of Iago's Soliloquy Analysis, β-alanine production were 11.95 and 13.4 μmol min −1 mg −1 protein, respectively.

Substrate conversion after 15 min was 1217% and 3144%, respectively, for the ABAL- and APAL-dependent reactions. Writing Service? 3.4 Subcellular localization of apple AMADHs. Amino acid sequence analysis revealed that the The Benefits of Hot Water Md AMADHs, as well as At ALDH10A9, Os AMADH1, Os AMADH2, Zm AMADH1a, Zm AMADH1b, Zm AMADH2, Hv AMADH1, Ps AMADH1, Ps AMADH2 and writing, Sl AMADH2 proteins possess a C-terminal canonical peroxisomal targeting signal 1 (PTS1, SKL), whereas At ALDH10A8, Hv AMADH2, So BADH and Sl AMADH1 do not (Supplementary Fig. S1). At ALDH10A8 is reported to localize to the leucoplast[16], whereas So BADH and Hv BBD2 are plastidial and cytosolic, respectively[18,33]. The subcellular location of Sl AMADH1 has not been studied. To examine the subcellular localization of apple AMADHs, Arabidopsis protoplasts were transformed with GFP-fusion constructs and organellar markers such as mCherry peroxisomal and RFP cytosolic were employed. The subcellular locations of the GFP-tagged AMADHs were determined by confocal laser-scanning microscopy. In all cases, both C- and N-terminal GFP fusion constructs were made to exclude the possibility that localization was dependent on the position of the difference between undergraduate uk GFP tag.

GFP- Md AMADH1 did not co-localize with the mCherry peroxisomal marker (Fig. 4ad), whereas GFP- Md AMADH2 displayed punctate structures and partially co-localized with the mCherry peroxisomal marker (il). This finding demonstrated that members of the apple AMADHs are present in two different subcellular compartments, the cytosol and the peroxisome, as described above for several plant species. Service? This might indicate non-redundant functions for the Md AMADHs at the protein level. Essay Sexuality Film Vs. Print? Md AMADH1-GFP and service, Md AMADH2-GFP co-expressed with RFP cytosolic were exclusively localized in cytosol (Fig. 4eh and mp, respectively). Similar behavior has been observed with C-terminal GFP fusion constructs of Md AO1[7]. Essay About With A Human? This suggests that the targeting role of the thesis writing C-terminus PTS1 is In Beowulf: vs. Print, masked in the Md AMADH1-GFP and Md AMADH2-GFP fusion proteins by writing, the GFP tag. Subcellular localization of Md AMADH1 and Md AMADH2 fusion proteins in Soliloquy Essay Arabidopsis cell suspension protoplasts.

Confocal laser scanning microscopic images of a protoplast transformed simultaneously with plasmid expressing either GFP- Md AMADH1 (a), Md AMADH1-GFP (e), GFP- Md AMADH2 (i) or Md AMADH2-GFP (m) (i.e., the first column) and mCherry peroxisomal marker (b and j) or RFP cytosolic marker (f and n) (i.e., the second column). A merged image of the thesis writing service GFP and mCherry or RFP fluorescence images is about Interview With a Human Resources, shown in service the third column (c, g, k and creole man, o) and co-localization of green and red fluorescence signals appears yellow. Cell morphology was observed with transmitted light microscopy and is shown in fourth column (d, h, i and thesis writing service, p). Images were taken 16 h after transformation. Essay Interview A Human Resources Manger? The experiment was repeated three times with similar results. Bars represent 10 μm. GABA can be derived via the oxidative deamination of polyamines in addition to thesis, the GABA-shunt pathway[6]. A pharmacological approach estimated that the terminal oxidation of Essay Sexuality Of Women In Beowulf: vs. Print, putrescine accounts for 39% of the thesis writing GABA concentration in roots of soybean seedlings exposed to creole man, salt stress[34]. There is thesis writing, a paucity of between uk, information about the role of polyamine oxidation in thesis service GABA biosynthesis in fruit[35]. Recently, Zarei et al.[7] described an difference uk apple fruit Md AO1 that is peroxisomal and has high catalytic efficiency for thesis writing 1,3-diaminopropane and putrescine to creole man, produce APAL and ABAL, respectively. In the present study, we identified two members of the ALDH10 protein family which encode apple AMADHs.

The pH optimum for Md AMADH1 and Md AMADH2 was 9.8. These findings are consistent with the pH optima (9.410.2) of plant AMADHs that have been previously characterized (i.e., Zm AMADH1a, Zm AMADH2, Sl AMADH2, Ps AMADH1, Ps AMADH2, Sl AMADH1, Zm AMADH1b and thesis service, Hv BBD1)[13,33]. It has been proposed that the Iago's Soliloquy Analysis Essay catalytic C294 ( Md AMADH numbering) preserves its thiolate form when pH is significantly above 8 to accelerate nucleophilic attack at the substrate[13]. The apple AMADHs studied here favored NAD + as the cofactor over NADP + with saturating APAL. Previously, Kope#x10d;ný et al.[13] reported that the E188 residue (pea, apple or Arabidopsis AMADH numbering) is thesis writing, responsible for NAD + preference (Supplementary Fig. S1), indicating that the apple AMADHs are probably NAD + -dependent enzymes. The apple AMADHs had negligible activities with BAL, findings similar to those previously reported for Zm AMADH1b, Zm AMADH2, Sl AMADH2 and Ps AMADHs[13]. Notably, Muñoz-Clares and co-workers[14,15] demonstrated that residue position 441 (SoBADH numbering) must be occupied by Sexuality In Beowulf: Film vs. Print, A or C for efficient BAL oxidation. Writing? For example, the BAL-dependent activity of a So BADH A441I mutant is only 0.5% of that for difference between undergraduate and postgraduate the wild-type protein. This position (residue 444 based on numbering of the apple and Arabidopsis AMADHs) is generally occupied by an Ile residue in plant AMADHs (Supplementary Fig. S1); exceptions are Zm AMADH1a, Hv AMADH2 and thesis service, So BADH, which have high affinity for BAL and where I is Water, replaced by thesis service, C, C and A, respectively[14,15], suggesting that the apple ALDH10 family proteins studied here should not be very active, if at all, with BAL.

In the present study, we demonstrated that in undergraduate vitro APAL was preferred over service, ABAL as a substrate (Table 1 andFig. 3). However, the Iago's Soliloquy Essay compartmentation and pH optimum of the enzyme, as well as availability of the alternative substrates, would be important determinants in the functional role of the thesis writing service enzyme in Why The vivo. Notably, the use of multiple substrates (i.e., substrate ambiguity) by an enzyme is becoming a better recognized phenomenon, which is thought to enhance the performance of cellular functions[36]. Subcellular localization studies of apple AMADHs were conducted using Arabidopsis cell suspension protoplasts. A majority of writing, plant AMADHs is targeted to Interview Resources Manger, the peroxisome, as indicted by the presence of the C-terminal PTS1, but there is also evidence for plastidial and cytosolic localizations[16,18,33].

The presence of canonical PTS1 (SKL) suggests that Md AMADH1 and Md AMADH2 would localize to thesis, the peroxisome. Surprisingly, the Why The Essay expressed Md AMADH1 fusion protein appeared to reside in the cytosol, whereas Md AMADH2 was partially targeted to the peroxisome (Fig. 4 ). Notably, both proteins carry identical canonical PTS1 (SKL) and residues in close proximity. Ma and Reumann[37] demonstrated that four and more acidic residues located in writing service close proximity to SKL (10 amino acid upstream of SKL) are incompatible with peroxisomal targeting, altering SKL to a non-functional PTS1. Essay Of Women In Beowulf:? The apple AMADHs possess two acidic residues in thesis service close proximity. This may explain partial localization of Md AMADH2 in the peroxisome, although the minimum number of Essay Sexuality Of Women In Beowulf: vs. Print, acidic residues that are required to hinder peroxisome targeting by thesis writing service, SKL has not been determined[37]. Difference Between Undergraduate Uk? Unpredictable masking of writing service, PTS1 surface exposure and competition between targeting signals are potential explanations for Essay Of Women In Beowulf: Film vs. Print a non-functional SKL which could prevent Md AMADH1 from reaching the peroxisome.

Recently, it has been reported that peroxisomal proteins often exhibit dual localization and this phenomenon is more widespread than previously thought[38]. Cytosolic AMADH1 could be retained in thesis the cytosol by various mechanisms such protein-protein interaction, protein conformational change in response to pH, or other post-translational modifications, and then released and targeted to the organelle via stress-specific signal perception[39]. This hypothesis would be in agreement with the marked difference between the slightly acidic pH of cytosol and the strongly alkaline pH optima of AMADHs, and with the targeting of the N-terminus and The Benefits of Hot Water Heaters, full-length protein of At ALDH10A8 to leucoplasts and thesis writing, cytosol, respectively[16]. Difference Between And Postgraduate Uk? This point must be clarified by thesis, future study. Supplementary data associated with this article can be found, in the online version, athttp://dx.doi.org/10.1016/j.febslet.2015.08.005. Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.febslet.2015.08.005. Creole Man? This research was supported by funds to BJS from the Individual Discovery Grants Program of the writing service Natural Sciences and on The Film, Engineering Research Council () of Canada.

The authors thank Dr. Thesis? Gordon J. Hoover for conducting the amino acid analysis, Dr. Robert T. Mullen for providing the fluorescent protein-containing vectors, the ALDH nomenclature committee for designating systematic names for our apple genes, and an anonymous reviewer for providing excellent comments on an earlier version of the manuscript. Supplementary data associated with this article can be found, in the online version, athttp://dx.doi.org/10.1016/j.febslet.2015.08.005. FEBS Letters 589 (2015) 1873-3468 2015 Federation of European Biochemical Societies. ABAL; 4-aminobutanal; ALDH; aldehyde dehydrogenase; AMADH; aminoaldehyde dehydrogenase; AO; amine oxidase; APAL; 3-aminopropanal; BAL; betaine aldehyde; GABA; 4-aminobutyrate; GFP; green fluorescent protein; NAD(P) + ; nicotinamide adenine dinucleotide (phosphate); PTS1; peroxisomal targeting signal 1; RFP; red fluorescent protein; 4-Aminobutyrate; 4-Aminobutanal; Aminoaldehyde dehydrogenase; Peroxisomal targeting signal; Polyamine; Apple. Issue online: 18 August 2015 Version of record online: 18 August 2015 Manuscript Accepted: 6 August 2015 Manuscript Revised: 4 August 2015 Manuscript Received: 25 May 2015. Supplementary information. Supplementary Materials and Methods, Figs. S1S3 and Table S1. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors.

Any queries (other than missing content) should be directed to the corresponding author for on The Sexuality In Beowulf: Film the article. 1 B.J. Shelp , A.W. Bown , M.D. McLean , Metabolism and functions of thesis, gamma-aminobutyric acid . Trends Plant Sci. , 4 , ( 1999 ), 446 452 . CrossRef | PubMed | Web of Science® Times Cited: 366 2 B.J. Shelp , W.L. Allan , D. Faure , Role of Essay Resources, γ-aminobutyrate and γ-hydroxybutyrate in service plant communication . F. Balu#x161;ka Plant-Environment Interactions ( 2009 ), Springer Heidelberg 73 84 . CrossRef 3 B.J.

Shelp , G.G. Bozzo , A. Zarei , J.P. Simpson , C.P. Trobacher , W.L. Allan , Strategies and tools for difference undergraduate studying the metabolism and thesis, function of γ-aminobutyrate in plants. Essay Interview A Human Resources? II. Integrated analysis . Botany , 90 , ( 2012 ), 781 793 . CrossRef | CAS | Web of Science® Times Cited: 15 4 B.J. Shelp , G.G. Writing? Bozzo , C.P. Trobacher , G. Chiu , V.S.

Bajwa , Strategies and between uk, tools for thesis studying the metabolism and function of γ-aminobutyrate in plants. I. Pathway structure . Botany , 90 , ( 2012 ), 651 668 . Analysis? CrossRef | CAS | Web of Science® Times Cited: 17 5 C.P. Trobacher , A. Zarei , J. Service? Liu , S.M. Clark , G.G. Bozzo , B.J. Essay About Interview With Resources Manger? Shelp , Calmodulin-dependent and calmodulin-independent glutamate decarboxylases in apple fruit . BMC Plant Biol. , 13 , ( 2013 ), 144 CrossRef | PubMed | CAS | Web of writing, Science® Times Cited: 9 6 B.J. Shelp , G.G. Bozzo , C.P. Trobacher , A. Interview Manger? Zarei , K.L.

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Shelp , Apple fruit copper amine oxidase isoforms: peroxisomal MdAO1 prefers diamines as substrates, whereas extracellular MdAO2 exclusively utilizes monoamines . Plant Cell Physiol. , 56 , ( 2015 ), 137 147 . Thesis Service? CrossRef | PubMed | Web of creole man, Science® Times Cited: 1 8 J. Planas-Portell , M. Gallart , A.F. Tiburcio , T. Altabella , Copper-containing amine oxidases contribute to writing service, terminal polyamine oxidation in between undergraduate and postgraduate peroxisomes and apoplast of Arabidopsis thaliana . BMC Plant Biol. , 13 , ( 2013 ), 109 CrossRef | PubMed | CAS | Web of thesis writing service, Science® Times Cited: 22 9 H. Flores , P. Filner , Polyamine catabolism in higher plants: characterization of pyrroline dehydrogenase . Plant Growth Regul. Difference Undergraduate And Postgraduate? , 3 , ( 1985 ), 277 291 . CrossRef | CAS | Web of thesis writing service, Science® Times Cited: 97 10 B. Weilgat , K. Kleczkowski , Putrescine in pea seedlings . Acta Soc. Bot. Pol. , 40 , ( 1971 ), 197 207 . CrossRef 11 V. Vasiliou , D.C. Thompson , C. Smith , M. Fujita , Y. Difference And Postgraduate Uk? Chen , Aldehyde dehydrogenases: from eye crystallins to metabolic disease and cancer stem cells . Chem. Biol. Interact. , 202 , ( 2013 ), 2 10 . CrossRef | PubMed | CAS | Web of thesis writing, Science® Times Cited: 19 12 H. Riveros-Rosasa , L. González-Segura , A. Julián-Sánchez , Á.G. Díaz-Sánchez , R.A. Muñoz-Clares , Structural determinants of substrate specificity in aldehyde dehydrogenases . Chem. Biol. Interact. , 202 , ( 2013 ), 51 61 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 11 13 D. Difference Undergraduate Uk? Kope#x10d;ný , R. Kon#x10d;itíková , M. Thesis? Tylichová , A. Vigouroux , H. The Benefits Water Heaters Essay? Moskalíková , M. Soural , M. #x160;ebela , S. Moréra , Plant ALDH10 family: identifying critical residues for substrate specificity and trapping a thiohemiacetal intermediate . Thesis Writing? J. Biol.

Chem. , 288 , ( 2013 ), 9491 9507 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 7 14 Á.G. Díaz-Sánchez , L. Civil? González-Segura , C. Mújica-Jiménez , E. Rudiño-Piñera , C. Montiel , L.P. Martínez-Castilla , R.A. Muñoz-Clares , Amino acid residues critical for specificity for betaine aldehyde of the plant ALDH10 isoenzyme involved in the synthesis of glycine betaine . Plant Physiol. , 158 , ( 2012 ), 1570 1582 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 19 15 R.A. Muñoz-Clares , H. Thesis? Riveros-Rosas , G. The Benefits? Garza-Ramos , L. González-Segura , C. Mújica-Jiménez , A. Julián-Sánchez , Exploring the evolutionary route of the acquisition of betaine aldehyde dehydrogenase activity by plant ALDH10 enzymes: implications for the synthesis of the osmoprotectant glycine betaine . BMC Plant Biol. , 14 , ( 2014 ), 149 CrossRef | PubMed | CAS | Web of Science® Times Cited: 2 16 T.D. Missihoun , J. Schmitz , R. Klug , H.H.

Kirch , D. Bartels , Betaine aldehyde dehydrogenase genes from Arabidopsis with different sub-cellular localization affect stress responses . Planta , 233 , ( 2011 ), 369 382 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 37 17 L.M. Service? Bradbury , S.A. Gillies , D.J. Brushett , D.L. Waters , R.J. Iago's Soliloquy Analysis? Henry , Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice . Writing Service? Plant Mol. War Started?? Biol. , 68 , ( 2008 ), 439 449 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 61 18 P. Thesis Writing Service? Weigel , E.A. Civil War Started?? Weretilnyk , A.D.

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Technol. , 75 , ( 2013 ), 106 113 . CrossRef | CAS | Web of Essay about With Resources, Science® Times Cited: 12 21 K.J. Livak , T.D. Schmittigen , Analysis of relative gene expression data using real-time quantitative PCR and the 2(−delta delta C ( T )) method . Methods , 25 , ( 2001 ), 402 408 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 39019 22 P. Pouparda , L. Parisib , C. Thesis? Campiona , S. Ziadia , P. Simoneaua , A wound- and ethephon-inducible PR-10 gene subclass from apple is differentially expressed during infection with a compatible and an incompatible race of Venturia inaequalis . Physiol. Mol. Plant Pathol. , 62 , ( 2003 ), 3 12 . CrossRef | CAS | Web of Why The War Started?, Science® Times Cited: 22 23 T. Writing? Czechowski , M. Stitt , T. Altmann , M.K. Udvardi , W.R. Scheible , Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis . Difference Between Undergraduate? Plant Physiol. Writing? , 139 , ( 2005 ), 5 17 . War Started? Essay? CrossRef | PubMed | CAS | Web of Science® Times Cited: 1134 24 T. Maniatis , E.F.

Fritsch , J. Sambrook , Molecular Cloning: A Laboratory Manual . 2nd ed. ( 1989 ), Cold Spring Harbor Laboratory Cold Spring Harbor, New York 25 W.L. Allan , B.J. Thesis? Shelp , Fluctuations of γ-aminobutyrate, γ-hydroxybutyrate, and related amino acids in With a Human Arabidopsis leaves as a function of the light-dark cycle . Thesis Writing Service? Can. J. Bot. , 84 , ( 2006 ), 1339 1346 . CrossRef | CAS | Web of Science® Times Cited: 20 26 A. Zarei , A.P. Körbes , P. Younessi , G. Montiel , A. Champion , J. Memelink , Two GCC boxes and AP2/ERF-domain transcription factor ORA59 in jasmonate/ethylene-mediated activation of the PDF1.2 promoter in Arabidopsis . Plant Mol. Biol. , 75 , ( 2011 ), 321 331 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 58 27 S.L.

Ching , S.K. Gidda , A. Rochon , O.R. van Cauwenberghe , B.J. On The Of Women Film? Shelp , R.T. Mullen , Glyoxylate reductase isoform 1 is writing, localized in the cytosol and Iago's Soliloquy Analysis Essay, not peroxisomes in plant cells . J. Integr. Plant Biol. , 54 , ( 2012 ), 152 168 . Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 10 28 J.M. Shockey , S.K. Gidda , D.C.

Chapital , J.C. Kuan , P.K. Thesis Service? Dhanoa , J.M. Bland , S.J. Rothstein , R.T. Mullen , J.M. Dyer , Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to Essay With, different subdomains of the endoplasmic reticulum . Plant Cell , 9 , ( 2006 ), 2294 2313 . CrossRef | CAS | Web of thesis writing service, Science® Times Cited: 212 29 M. Axelos , C. Creole Man? Gurie , L. Mazzolini , C. Bardet , B. Lescure , A protocol for transient gene expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures . Plant Physiol. Biochem. , 30 , ( 1992 ), 123 128 . Web of Science® Times Cited: 267 30 J. Schirawski , S. Planchais , A.L. Haenni , An improved protocol for writing the preparation of difference between undergraduate, protoplasts from an established Arabidopsis thaliana cell suspension culture and infection with RNA of turnip yellow mosaic tymovirus: a simple and reliable method . Service? J. Virol. Creole Man? Methods , 86 , ( 2000 ), 85 94 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 28 31 M. Tylichová , D. Kope#x10d;ný , S. Moréra , P. Briozzo , R. Writing Service? Lenobel , J. Snégaroff , M. Sexuality Of Women In Beowulf: Film Vs. Print? #x160;ebela , Structural and functional characterization of plant aminoaldehyde from Pisum sativum with a broad specificity for thesis service natural and synthetic aminoaldehydes . J. Mol. Creole Man? Biol. Thesis? , 396 , ( 2010 ), 870 882 . Creole Man? CrossRef | PubMed | CAS | Web of Science® Times Cited: 24 32 D. Kope#x10d;ný , M. Tylichová , J. Snegaroff , R. Kon#x10d;itíková , H. Popelková , M. #x160;ebela , Carboxylate and thesis writing, aromatic active-site residues are determinants of high affinity binding of ω-aminoaldehydes to plant aminoaldehyde dehydrogenase . FEBS J. , 278 , ( 2011 ), 3130 3139 . Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 11 33 T. Fujiwara , K. Hori , K. Ozaki , Y. Yokota , S. Mitsuya , T. Ichiyanagi , T. Essay On The In Beowulf:? Hattori , T. Thesis Writing Service? Takabe , Enzymatic characterization of peroxisomal and cytosolic betaine aldehyde dehydrogenase in barley . Physiol.

Plant. , 134 , ( 2008 ), 22 30 . Wiley Online Library | PubMed | CAS | Web of Science® Times Cited: 33 34 S.G. Xing , Y.B. Jun , Z.W. Hau , L.Y. Iago's Soliloquy? Liang , Higher accumulation of #x263;-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidase in Glycine max (L.) Merr. Thesis Writing? roots . Plant Physiol. Biochem. , 45 , ( 2007 ), 560 566 . CrossRef | PubMed | CAS | Web of about Interview With Resources, Science® Times Cited: 58 35 K.L. Deyman , C.J. Brikis , G.G. Thesis Writing Service? Bozzo , B.J. Shelp , Impact of 1-methylcyclopropene and controlled atmosphere storage on polyamine and 4-aminobutyrate levels in ‘Empire’ apple fruit . Front.

Plant Sci. , 5 , ( 2014 ), 144 CrossRef | PubMed | Web of Science® Times Cited: 4 36 C. Pandya , J. Creole Man? Farelli , D. Dunaway-Mariano , K.N. Allen , Enzyme promiscuity: engine of evolutionary innovation . J. Thesis Writing Service? Biol. Chem. , 289 , ( 2014 ), 30229 30236 . Why The Essay? CrossRef | PubMed | CAS | Web of Science® Times Cited: 15 37 C. Ma , S. Reumann , Improved prediction of peroxisomal PTS1 proteins from genome sequences based on experimental subcellular targeting analyses as exemplified for protein kinases . J. Exp. Bot. Thesis? , 59 , ( 2008 ), 3767 3779 . CrossRef | PubMed | CAS | Web of Science® Times Cited: 24 38 J. Ast , A.C. Stiebler , J. Freitag , M. Bölker , Dual targeting of about With, peroxisomal proteins . Front. Physiol. , 4 , ( 2013 ), 297 CrossRef | PubMed | Web of Science® Times Cited: 17 39 M.C.

Cheng , E.J. Hsieh , J.H. Chen , H.Y. Chen , T.P. Lin , Arabidopsis RGLG2, functioning as a RING E3 ligase, interacts with AtERF53 and thesis, negatively regulates the plant drought stress response . Civil? Plant Physiol. , 158 , ( 2012 ), 363 375 . Thesis Service? CrossRef | PubMed | CAS | Web of Science® Times Cited: 41. Articles related to the one you are viewing. 2017 Federation of European Biochemical Societies.

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resume withdrawal Protracted Withdrawal Syndromes. Journal of Substance Abuse Treatment. Vol. Thesis Writing! 8 pp. 19-28 1991. Professor C Heather Ashton DM, FRCP. School of of Hot Heaters, Neurosciences. Division of Psychiatry. The Royal Victoria Infirmary.

Queen Victoria Road. Newcastle upon service, Tyne NE1 4LP. Abstract: The benzodiazepine withdrawal syndrome is a complex phenomenon which presents serious difficulties in definition and measurement. It is between undergraduate and postgraduate particularly difficult to set out precise limits on thesis writing service its duration. Many withdrawal symptoms are a result of pharmacodynamic tolerance to benzodiazepines, some mechanisms for which are discussed. Such tolerance develops unevenly in different brain systems and may be slow to Water Heaters Essay reverse. Withdrawal symptoms occurring in the first weeks after cessation of drug use tend to merge with more persistent symptoms that may last for many months. These prolonged symptoms do not necessarily constitute true pharmacological withdrawal symptoms, but are nevertheless related to long-term benzodiazepine use. Such symptoms can include anxiety, which may partly result from a learning deficit imposed by the drugs, and a variety of sensory and motor neurological symptoms. The protracted nature of thesis service, some of these symptoms raises the possibility that benzodiazepines can give rise not only to Iago's slowly reversible functional changes in thesis service the central nervous system, but may also occasionally cause structural neuronal damage. Keywords: benzodiazepines; withdrawal syndrome; tolerance; protracted symptoms; tinnitus; brain mechanisms.

Drug withdrawal syndromes, in general, tend to consist of Iago's Soliloquy Analysis, mirror images of the drugs' initial effects. Thus, abrupt withdrawal from chronic usage of beta adrenoceptor antagonists such as propranolol may give rise to tachycardia and palpitations; abrupt withdrawal from antihypertensive doses of clonidine may be followed by hypertension, anxiety, and other signs of increased sympathetic activity. Thesis Writing Service! Benzodiazepines are no exception: On sudden cessation after chronic use, anticonvulsant effects may be replaced by epileptic seizures, muscle relaxation by increased muscle tension, hypnotic effects by increased anxiety. The same symptoms can occur in attenuated form when the drugs are withdrawn slowly. However, all of these symptoms are not inevitable in any individual patient. The particular features of the withdrawal syndrome and their time of onset, duration, and severity are greatly modified by many other factors. Such factors include pharmacokinetic variables, dosage and duration of drug use, rate of withdrawal, the presence or absence of the original disorder (such as anxiety) for which the drug was prescribed, personality characteristics, physical makeup and susceptibility, and the use of concomitant treatments. These variables alone make it difficult to characterise specific features of the The Benefits of Hot Essay, withdrawal syndrome. This difficulty is compounded by the fact that, as long-term medication, benzodiazepines have mainly been prescribed for anxiety and insomnia, disorders which themselves include most features of the drug withdrawal syndrome.

When such patients undergo reduction of benzodiazepine dosage, especially slow reduction, how can one specify which emergent symptoms are true, drug-related withdrawal symptoms, which are pseudowithdrawal symptoms (Tyrer, Owen, Dawling, 1983), which represent a return of the writing, original anxiety state, and which are the natural reactions of an anxious personality undergoing the stress of creole man, withdrawal? In circumstances such as these, the benzodiazepine withdrawal syndrome becomes largely a matter of definition. Nevertheless, the writing service, existence of a benzodiazepine withdrawal reaction, from undergraduate both high and low (therapeutic) doses of benzodiazepines, is no longer in dispute, and many attempts have been made to define and thesis writing service measure it and to estimate its incidence and duration. Definitions and Measurements. Symptoms occurring during benzodiazepine withdrawal have been described by many authors (Ashton, 1984, 1987; Busto, Sellers, Naranjo, Cappell, Sanchez, Sykora, 1986; Hallström Lader, 1981; Murphy, Owen Tyrer, 1983, 1984; Petursson Lader, 1981a, 1981b; Smith Wesson, 1983; Tyrer et al ., 1983; Tyrer, Rutherford, Higgett, 1981; Winokur, Rickels, Greenblatt, Snyder, Schatz, 1980; among others). Commonly described symptoms are shown in Figure 1. None of these symptoms are specific to benzodiazepine withdrawal: They include all of the psychological and somatic symptoms of about With a Human Resources Manger, anxiety, although certain symptom clusters are characteristic. Service! Owen and Tyrer (1983) and Petursson and Lader (1981a b) emphasised the appearance of new symptoms, not experienced before withdrawal and creole man uncommon in anxiety states.

These new symptoms include hypersensitivity to sensory stimuli (sound, light, touch, taste, and smell) and perceptual distortions (e.g., sensation of the floor undulating, feeling of service, motion, impression of walls or floor tilting). There also appears to be a higher incidence than usually seen in anxiety of depersonalisation, derealisation, paraesthesiae, and extreme dysphoria, an amalgam of anxiety, depression, nausea, malaise, and depersonalisation (Petursson Lader, 1981a, 1981b). Visual hallucinations, distortion of body image, psychotic reactions, formication, muscle fasciculation and twitching (occasionally resembling myoclonus), and considerable loss of weight are also described during benzodiazepine withdrawal and are unusual in anxiety states. Smith and With a Human Wesson (1983) and Ashton (1984) drew attention to the characteristic fluctuation of thesis, symptoms, which may wax and wane without obvious psychological provocation. Smith and Wesson (1983) suggest that this wavelike symptomatology is an important marker for distinguishing the Essay on The Of Women In Beowulf:, low-dose benzodiazepine withdrawal syndrome from symptom re-emergence. However, since symptoms may fluctuate in the course of the day or over periods of writing service, days or weeks, accurate recording is difficult. Since no particular symptom is exclusive to creole man benzodiazepine withdrawal, how can one define the thesis service, syndrome? Tyrer et al . (1981, 1983) have attempted various methods in placebo controlled studies: The appearance of two or more new symptoms during the withdrawal period. Of Hot Water Heaters Essay! New symptoms in one study included perceptual disturbances, sensory hypersensitivity, and fear of imminent death (1981), and in thesis another study (1983), psychotic symptoms, depression and dysphoria, muscle twitching, and abnormal sensations of movement. The choice of two new symptoms as the Essay, minimum number necessary to qualify as withdrawal is clearly arbitrary.

An increase in writing self-rated symptoms (Comprehensive Psychiatric Rating Scale) to greater than 50% of baseline levels, followed by The Benefits of Hot Essay a return to lower values. Symptom resolution is clearly an important feature in differentiating between symptoms due to withdrawal and symptom re-emergence, emergence, or overinterpretation. As Smith and service Wesson (1983, p. Between Undergraduate Uk! 88) point out: Withdrawal symptoms subside with continued abstinence, whereas symptoms of writing service, other aetiology persist. However, a measure of creole man, symptom resolution is thesis not applicable to patients who drop out of withdrawal studies, perhaps because of intolerable true withdrawal symptoms. The Benefits Water! It is noteworthy that 45% of patients dropped out of one study (Tyrer et al ., 1981).

A combination of methods (1) and (2) so that a withdrawal reaction is defined as the appearance of new symptoms that resolved before the thesis, end of the study (20 and Essay Film vs. Print 14 weeks after the end of withdrawal [Tyrer et al ., 1983]). More recently Tyrer, Murphy, Riley (1989) have produced a questionnaire of symptoms that are relatively specific to thesis writing service benzodiazepine withdrawal in that they mainly occur during periods of drug withdrawal and return towards baseline levels after withdrawal. Pseudowithdrawal symptoms were defined as symptoms occurring when patients thought they were reducing but their drug consumption and blood concentrations of benzodiazepines were unchanged. Such definitions, derived from double-blind, placebo-controlled studies, have been extremely helpful in The Benefits Essay the recognition of benzodiazepine dependence, especially low-dose dependence. However, they are of necessity arbitrary and thesis can only Soliloquy, be approximate, since the appearance or severity of any particular symptom or symptom cluster may actually represent a variable combination of true withdrawal, pseudowithdrawal, and re-emergence of anxiety, and the same patients liable to writing service pseudowithdrawal reactions are also likely to be most vulnerable to The Benefits Water true withdrawal effects. Furthermore, definitions based on differences from prewithdrawal symptoms do not take into thesis account the possibility that, due to the development of tolerance, withdrawal symptoms may already be present while patients are still taking benzodiazepines. Such a situation is most clearly seen with relatively short-acting benzodiazepines. For example, patients taking triazolam as a hypnotic commonly develop daytime anxiety (Oswald, 1989) and Why The Civil War Started? even hallucinations or psychotic reactions. These are almost certainly withdrawal effects since they are immediately relieved by thesis writing service taking the drug but eventually disappear after the drug is stopped (Ashton, 1987). Similarly, with lorazepam and alprazolam (Hermann, Brotman, Rosenbaum, 1987) patients often develop increasing anxiety and panic as well as craving between doses (Ashton, 1984). They appear to Heaters Essay undergo a miniwithdrawal between each dose, which is temporarily relieved by the next tablet, but disappears after total cessation.

An analagous condition is seen with alcohol: alcoholics commonly complain of thesis writing, tremor and Heaters insomnia, symptoms which are temporarily relieved by alcohol but which only disappear after a period of abstinence. Thesis Writing Service! Even with long-acting benzodiazepines such as diazepam, there is Essay usually a history in long-term users of steadily increasing anxiety, with the writing, development over the years of new symptoms such as agoraphobia, often with perceptual distortions and depersonalisation, despite continued usage of these supposedly anxiolytic drugs. These symptoms have often been temporarily alleviated by a moderate increase in Sexuality In Beowulf: vs. Print dosage or the addition of another benzodiazepine, but eventually re-emerge during further chronic use and thesis service only disappear after the creole man, benzodiazepine is writing service stopped (Ashton, 1984, 1987). Mechanisms of tolerance and withdrawal symptoms are discussed below, but tolerance is difficult to demonstrate in clinical practice. Because of these many immeasurable factors, it is difference between doubtful whether the boundaries of a true benzodiazepine withdrawal syndrome can ever be clearly demarcated. The overall incidence of the benzodiazepine withdrawal syndrome is unknown.

As with cigarette smokers (Ashton Stepney, 1982), there may be a large, uncounted population who quit regular benzodiazepine usage after months or years without ever coming to medical attention. Tyrer (1990) notes that it is surprising how many patients in ordinary practice have no difficulties whatsoever in thesis writing service reducing their benzodiazepines, and the incidence of a benzodiazepine withdrawal syndrome in general practice appears to be around 30% (Tyrer et al ., 1981, 1983; Tyrer, 1989; Tyrer, Murphy, Riley, in press; Tyrer, 1990). On the other hand, in selected patients referred for specialist treatment, the incidence may be 100% (Ashton, 1987; Lader Olajide, 1987; Petursson Lader, 1981a). It is also worth noting that withdrawal syndromes in the form of rebound insomnia (Kales, Scharf, Kales, 1978) or more general symptoms can occur in experimental subjects and in nonanxious patients prescribed benzodiazepines for sports injuries (Lader, 1988). Essay About Interview With Resources Manger! In addition, a benzodiazepine withdrawal syndrome has been described in neonates whose mothers took therapeutic doses of benzodiazepines during pregnancy (Rementeria Bhatt, 1977). Not surprisingly, the thesis writing service, observed incidence of benzodiazepine withdrawal reactions depends not only on patient selection but also on the criteria used for measurement. About Resources Manger! In the study of thesis service, Tyrer et al . (1983), when definitions of withdrawal (1) and undergraduate and postgraduate (2) (see above) were used singly, the incidence of pseudowithdrawal reactions was around 20%.

With the writing, combined definition (3) (see above) the incidence of true withdrawal symptoms was 44% and there were no pseudowithdrawal reactions. This incidence of course only applies to those consenting to take part in the study and managing to finish it. It cannot account for dropouts during withdrawal or for individuals declining to undergo withdrawal (45.5% of Civil Essay, eligible patients in the study of thesis, Tyrer et al ., 1981). Thus the incidence of benzodiazepine withdrawal, like its diagnosis, becomes largely a matter of definition. The identification of the benzodiazepine withdrawal syndrome is difficult enough; its duration is even more difficult to delineate. Most estimates suggest a duration of difference between, approximately 5-28 days, with a peak in thesis severity around 2 weeks post withdrawal, after which most symptoms return to prewithdrawal levels (Busto et al ., 1986; Murphy et al ., 1984; Owen Tyrer, 1983; Petursson Lader, 1981a, 1981b; Tyrer et al ., 1981, 1983).

To a large extent the apparent duration depends upon how long the patients are followed up, and several authors have drawn attention to the prolonged nature of postwithdrawal symptoms in creole man some cases. For example, Smith and thesis Wesson (1983) observed that symptoms after withdrawal from low-dose benzodiazepine typically take 6-12 months to subside completely. Prolonged symptoms included anxiety, insomnia, paraesthesiae, altered sensation, muscle spasms, and psychosis. Ashton (1984, 1987) reported a similar protracted time-course. Tyrer (1990) refers to a post-withdrawal syndrome in the 6 months after withdrawal.

Hallström and Lader (1981) found the Hamilton Anxiety Score still raised above baseline levels by Sexuality In Beowulf: vs. Print follow-up several months later when successfully withdrawn patients had resumed their normal life (Hallström Lader, 1981 p. 237). Olajide and Lader (1984) suggested that depression may be an integral part of the benzodiazepine withdrawal syndrome and may last several months after withdrawal in thesis service susceptible individuals; this phenomenon was also observed by Ashton (1987). Busto, Fornazzari, and Naranjo (1988) described two cases in whom severe tinnitus first appeared during benzodiazepine withdrawal and persisted for 6 and 12 months after discontinuation before finally diminishing or disappearing. In one of these cases the tinnitus was immediately alleviated by diazepam in a double-blind placebo-controlled trial conducted over 1 week, 6 months after withdrawal; after a further 6 months of abstinence the tinnitus had became tolerable. In a recent study of 68 patients who were withdrawn from Soliloquy Analysis benzodiazepines over a 6-week period and followed for a further 4 weeks, Tyrer et al . (1989), using a self-report scale, found a wide variation in the time at which individual symptoms peaked.

Mean scores for some symptoms (depression, dizziness, paraesthesiae, feeling of unreality) peaked early, while mean scores for others (nausea, memory impairment, faintness, touch sensitivity, and thesis writing service motor impairment) were maximal 8 weeks after the start of withdrawal. Although individual patient's scores were not reported, and 30% withdrew from the study, these findings suggest that symptoms can persist beyond the creole man, 5-28 days usually regarded as the duration of the withdrawal syndrome. Ashton, Rawlins and Tyrer (1990) used the rating scale shown in writing Figure 1 to record the creole man, symptoms of patients undergoing diazepam withdrawal under double-blind placebo controlled conditions. Withdrawal took place over 4 weeks, and writing service patients were followed for 8 weeks after the end of With a Human, withdrawal. Writing! Half the patients received placebo and the other half withdrawal. Analysis of the time-course of selected symptoms in the 11 out of 12 patients in the placebo group who successfully completed withdrawal is shown in Table 1. Of Women In Beowulf: Vs. Print! Eight weeks after the end of withdrawal, mean scores for headache, dizziness, depression, tinnitus, paraesthesiae, and motor symptoms remained higher than prewithdrawal scores; other symptoms had declined although few had disappeared. These findings again show that duration of symptoms after benzodiazepine withdrawal is often a matter of months rather than weeks. Different symptoms persisted in the Tyrer et al . (1989) and Ashton et al . (1990) studies, which differed also in size of sample, patient selection, and rate of thesis service, withdrawal. To what extent such persistent symptoms are true withdrawal symptoms is between undergraduate unknown. A further problem in assessing the duration of the withdrawal syndrome is the thesis writing service, interpretation of the baseline (prewithdrawal) symptoms and Essay a Human Resources anxiety scores.

Patients presenting for benzodiazepine withdrawal often have high levels of anxiety and writing service many psychological and Essay on The Of Women Film vs. Print somatic symptoms. Figures 2 and 3 show Hospital Anxiety Depression (anxiety) (Zigmond Snaith, 1983) and symptom rating scores for 12 patients on thesis writing service benzodiazepines compared with the scores of 18 healthy university students approaching their exams. Both groups took placebo tablets and were followed for 20 weeks. Why The Civil War Started?! The benzodiazepine group withdrew from the benzodiazepines between weeks 8 and 12. It is clear that the patients had considerably higher scores than the normal subjects on both scales, even at the beginning of the study while they were still taking benzodiazepines. Certainly in these patients the benzodiazepines were not effectively controlling anxiety and, as argued above, it is possible that at least some of the presenting symptoms were due to withdrawal symptoms even in the presence of the thesis writing, drug, as a result of the development of drug tolerance. Such symptoms would be expected to disappear after withdrawal, but they could be slow to resolve. Creole Man! There was no evidence of resolution below baseline levels 8 weeks after withdrawal, but unfortunately (as in most studies) formal assessment stopped at service this point. However, continued clinical contact with most of these patients has shown that anxiety symptoms have declined over time. This slow improvement bears out the observations quoted above (Ashton, 1984, 1987; Busto et al ., 1988; Hallström Lader, 1981; Olajide and The Benefits Heaters Essay Lader, 1984; Smith Wesson, 1983) that symptoms improve gradually for many months after withdrawal, and thesis writing some patients are able to resume normal lives after years of incapacity before withdrawal. Which of these long-lasting symptoms can one attribute to true drug withdrawal effects?

Is it possible to pinpoint a time at on The Sexuality Film vs. Print which the benzodiazepine withdrawal syndrome ends and to say with certainty that any residual symptoms must be due to other factors? The problem is similar to that of service, designating which effects of a bout of influenza or infectious mononucleosis can be attributed to the specific virus. Civil War Started?! Do such effects include only those of the viral toxaemia? Do they include secondary bacterial infection consequent upon the impact of the thesis, virus? Do they include the period of creole man, postviral lethargy and depression which, like benzodiazepine withdrawal symptoms, tends to writing recur in Essay about a Human Resources wave-like fashion for several months? Once again, the benzodiazepine withdrawal syndrome appears to slip through the fingers and one is led back to a question of definition. Service! Probably a clear definition of duration is impossible because drug-induced perturbations of central neurotransmission merge imperceptibly into Essay Sexuality Of Women Film vs. Print the background of individual, genetically determined, and learned patterns of writing, brain activity. Protracted Symptoms After Benzodiazepine Withdrawal. All the problems of definition discussed above are multiplied in of Hot Water Heaters any attempt to describe protracted benzodiazepine withdrawal syndromes. Yet anyone observing patients for long periods after withdrawal cannot fail to be struck by the persistence of certain symptoms in thesis writing service some patients. These may not be true benzodiazepine withdrawal symptoms; nevertheless they are possibly related to benzodiazepine use and often present a clinical problem.

Listed below are examples of some protracted symptoms that from personal observations and other reports appear to be relatively common after benzodiazepine withdrawal. Unfortunately, there are no data available on Essay on The Film vs. Print the incidence and duration of such symptoms in thesis writing comparable patients not treated with benzodiazepines, nor of their relative incidence in patients undergoing benzodiazepine withdrawal. The Benefits Water! Nor are there any known predictive factors to indicate which patients might be especially vulnerable. Anxiety: As discussed above, anxiety may persist for many months after benzodiazepine withdrawal, yet slowly decline below prewithdrawal levels after 1-2 years (Ashton, 1987). One reason for the slow resolution may be that benzodiazepines inhibit the learning of stress-coping strategies.

Such effects have been demonstrated in animals and man (Gray, 1987). Consequently there is a long period after benzodiazepine withdrawal when patients have a decreased ability to cope with stressful situations (Ashton, 1989; Murphy Tyrer, 1988; Owen Tyrer, 1983). Recovery may require many months of learning new strategies of stress control to replace the years of coping with stress by means of exogenous drugs. Hence persisting anxiety after benzodiazepine withdrawal does not necessarily imply the re-emergence of an anxiety state existing before benzodiazepine treatment; it may represent the uncovering of a type of learning deficiency induced by long-term benzodiazepine use. People who take benzodiazepines tend to have high ratings for trait anxiety (Ashton and Golding, 1989; Golding Cornish, 1987; Golding, Harper, Brent-Smith, 1983) which may confer a particular vulnerability to the stress of thesis service, withdrawal. Nevertheless, even without formal treatment, protracted anxiety symptoms, including agoraphobia and panic, may gradually resolve after benzodiazepine withdrawal (Ashton, 1987) although the process may be hastened by behavioural treatments.

Depression: Depression can be caused or aggravated by chronic benzodiazepine use (Lader Petursson, 1981), yet it also appears to be a feature of the withdrawal syndrome (Olajide Lader, 1984). It may be severe enough to qualify as a major depressive disorder (Ashton, 1987) and may persist for Iago's Analysis, some months. Olajide and service Lader (1984) suggest that the mechanisms for Essay on The Film vs. Print, postwithdrawal depression may be central serotonin depletion, but there is no direct evidence for this. Clinically, the depression is not different from depressive illness in general, and it responds to antidepressant drugs. It is not clear whether protracted depressive symptoms are more common in patients with a previous history of service, depression or whether it recurs in subsequent years after withdrawal. Tinnitus: Tinnitus is a common symptom of benzodiazepine withdrawal and may initially result from the characteristic general hypersensitivity to sensory stimuli.

It usually resolves in a few weeks, but occasionally qualifies as a protracted symptom. Busto et al . Essay! (1988) describe two cases in which tinnitus persisted for writing, 6 and 12 months after withdrawal and mention a third patient who was unable to withdraw from benzodiazepines because of severe tinnitus at each attempt. Difference Between Undergraduate And Postgraduate Uk! Further cases of protracted tinnitus personally observed are described below. Case 1: Female, aged 54. Duration of thesis, benzodiazepine usage: 8 years. Iago's Essay! Withdrawal slowly from oxazepam 45mg daily in 1986. Thesis Writing! Right-sided tinnitus first noticed during previous attempts at withdrawal; became severe after final withdrawal and is still constantly present and severe 2 years later, remaining mainly unilateral. No abnormality detected on skull x-ray, CAT scan, EEG, or ENT examination, apart from creole man slight bilateral (symmetrical) high-tone deafness.

Not clinically anxious or depressed; all other withdrawal symptoms resolved quickly. Thesis Writing! Taking no medication. Case 2: Female, aged 62. Duration of benzodiazepine usage: 18 years. Withdrawn slowly from diazepam 15mg daily in 1988.

Bilateral tinnitus first noticed during previous attempts at withdrawal, becoming severe after final withdrawal, and still present, severe, and continuous 1 year later. ENT investigation: moderate bilateral high-tone deafness; wears hearing aid. No clinical anxiety or depression; no other withdrawal symptoms; no medication. Case 3: Female, aged 58. Creole Man! Duration of benzodiazepine usage: 20 years.

Withdrew from diazepam 20mg daily over 4 weeks in 1988. Writing! Developed acute psychotic reaction, which resolved in Why The War Started? one week. Writing! First noticed left-sided tinnitus 1 month after withdrawal. Three months later restarted diazepam 20mg daily because of Essay Interview, unremitting tinnitus, but experienced only thesis service, slight improvement. Second withdrawal over one year 1988-9. Left-sided tinnitus severe throughout withdrawal and still persisting though becoming intermittent.

ENT examination negative except for slight bilateral (symmetrical) high-tone deafness. Medication: mianserin 30mg daily, started after onset of Soliloquy Essay, tinnitus; carbamazepine recently added with no effect on tinnitus. Case 4: Female, aged 70. Duration of writing service, benzodiazepine usage: 20 years. First noticed tinnitus, mainly right-sided, on withdrawal from flurazepam 30mg daily in Iago's Soliloquy Essay 1985. Tinnitus continuous ever since, not relieved by thesis a course of diazepam. ENT examination normal. Drugs: no psychotropics; inhalers for asthma. Tinnitus is fairly common in the general population and Film the apparent relation to benzodiazepine use may be incidental, but these cases raise the suspicion that benzodiazepines may occasionally cause permanent or only slowly reversible brain damage. Such damage may not be detectable on CAT scans; one study (Lader Petursson, 1984) suggested a mild degree of thesis writing, cortical shrinkage in chronic benzodiazepine users, but this finding was not confirmed in of Hot Water Essay a later study (Perera, Powell, Jenner, 1987).

Two of the above patients also complained of unilateral headaches. Paraesthesiae: Paraesthesiae, in the form of tingling, pins and needles or numbness of the extremities or circumoral region, is another common symptom of benzodiazepine withdrawal. The symptom also occurs in anxiety and possibly results from hyperventilation. Benzodiazepines depress the sensitivity of the respiratory centre to carbon dioxide (Gilmartin, Corris, Stone, Veale, Gibson, 1988) and it is possible that the respiratory centre becomes hypersensitive during withdrawal, triggering hyperventilation. Resolution of paraesthesiae usually occurs within a few weeks of withdrawal. Occasionally, however, patients complain of thesis service, numbness or of a burning sensation affecting the fingers, feet, and legs that may be protracted for months or years.

The symptoms suggest a peripheral sensory neuropathy and there may be demonstrable sensory impairment to light touch. Between Undergraduate! Two patients summarised below typify several similar cases personally observed at thesis writing a benzodiazepine withdrawal clinic. Case 1: Female, aged 56. Duration of benzodiazepine usage: 15 years. Severe continuous burning pain of undergraduate, feet first noticed in thesis service 1984 during slow reduction of lorazepam 7.5mg to 1.5mg daily. Changed to diazepam and slowly withdrawn over 1 year 1987-8.

Burning feet persisted for a further year, gradually decreasing in Essay on The Sexuality Film vs. Print intensity and now almost disappeared. No abnormal signs; good peripheral pulses. Thesis Writing Service! Medication; promethazine 50mg nocte since 1988. Case 2: Female, aged 67. Duration of benzodiazepine usage: 12 years. First noticed severe burning pain in feet and legs in 1981 during reduction of medazepam 15mg to 5mg daily. Pain persisted during diazepam substitution and slow withdrawal in 1985. Why The War Started?! 1986: still severe burning pain in legs not relieved by chlordiazepoxide. 1989: pain still present.

Neurological examination, nerve conduction studies, serum foliate and B 12 levels normal; peripheral pulses present and normal. Formication is also common during benzodiazepine withdrawal, and many patients temporarily complain of thesis service, a feeling of insects crawling on the skin or of lice or nits in the hair. Occasionally, more bizarre sensations are reported, such as a feeling of slime or water running over the body, a sense of inner vibration, or a feeling of trembling inside, and these symptoms may be protracted. Such symptoms may be variants of the burning sensations described above or may possibly be psychotic, but have a temporal relationship to benzodiazepine withdrawal. Motor Symptoms: Increased muscle tension, hyperreflexia, tremor, fasciculation, and muscle jerking are common features of benzodiazepine withdrawal but usually resolve within weeks. Occasionally muscle jerking persists for on The In Beowulf:, a year or more after withdrawal, and thesis writing service the clinical picture may suggest myoclonus, tics, or exaggerated startle reactions. Shoulder girdle and limb muscles are usually affected, but blepharospasm can occur. Some patients complain of violent jerking of the whole body at the onset of sleep, which may occur 20 or more times a night. Restless legs syndrome may also be protracted.

Some of these symptoms may result from increased peripheral sympathetic tone since they are often improved by propranolol. Others may be centrally generated and at least partially respond to Essay about Resources Manger carbamazepine. Blepharospasm can be controlled by local injection of botulinum toxin at 3 monthly intervals. Protracted motor symptoms such as these raise the possibility that benzodiazepines are capable of causing long-term hyperexcitability of motoneurons or central motor pathways. Gastrointestinal Symptoms: Gastrointestinal symptoms are extremely common during chronic benzodiazepine use and in withdrawal. Many chronic benzodiazepine users have been investigated by gastroenterologists and found to writing have irritable bowel syndrome (Ashton, 1987).

Gastrointestinal symptoms may be aggravated by hyperventilation (Lum, 1987) and may disappear completely after benzodiazepine withdrawal, even in patients who have had irritable bowel syndrome for Water, years. Nevertheless, there remains a sizeable core of patients who complain of food intolerance and gaseous abdominal distension which first appears during withdrawal and is protracted for many months. Tests for specific food allergies almost always prove negative, and the condition is unresponsive to conventional treatment. Patients often turn to alternative or fringe medicine, undergo various forms of diet, and become convinced that they have intestinal candidiasis or damage to the immune system. None of these claims have scientific support, although Lum (1987) reports that hyperventilation provokes histamine release and thesis service that the incidence of food intolerance and In Beowulf: pseudo-allergic reactions is high in chronic hyperventilators. Thesis Writing! The effect of benzodiazepine withdrawal on gastrointestinal function and on coriocosteroid and immune responses (known to be affected by stress) perhaps merits further attention.

Brain Mechanisms of Iago's Soliloquy Analysis, Benzodiazepine Withdrawal Symptoms. The primary effect of thesis writing service, benzodiazepines is enhancement of gamma-aminobutyric acid (GABA) activity on of Hot postsynaptic GABA-A receptors in the brain. The effect results from an interaction with specific benzodiazepine binding sites on the GABA-receptor complex (Möhler Okada, 1977; Squires Braestrup, 1977), which increases the affinity of the receptors for GABA (Costa, 1981). GABA neurons consist of small interneurons forming local circuits which exert a powerful influence on writing the excitability of other neurons passing through their spatial domain (Bloom, 1985). Such local GABA circuits are widely distributed throughout the brain, including the reticular formation, limbic system structures, cerebral and cerebellar cortex (Young Kuhar, 1980). GABA is difference undergraduate and postgraduate a universal inhibitor of writing service, nervous activity and also inhibits the release of excitatory neurotransmitters (Benton Rick, 1976).

Thus the actions of benzodiazepines include not only enhancement of GABA activity at Essay many brain sites but also decreased release of acetylcholine, noradrenaline, dopamine and serotonin (Faefely, Pieri, Pole, Schaffer, 1981). The clinical effects of thesis writing service, benzodiazepines probably result from a combination of these primary and secondary effects at on The Film vs. Print critical sites. For example, the writing, anxiolytic effects may be due to decreased serotonergic and noradrenergic activity in septo-hippocampal pathways (Gray, 1981). Thus benzodiazepine actions are by no means confined to a particular neurotransmitter or brain pathway. Any chronically used drug gradually engenders a series of Why The War Started?, homeostatic responses which tend to restore normal function despite the presence of the drug.

With chronic benzodiazepine use, compensatory changes occur in GABA receptors. Such changes consist of decreased sensitivity of these receptors to thesis GABA, probably as a result of alterations in affinity state and decreased density (Cowan Nutt, 1982; Nutt, 1986). Interview! In addition, there are changes in the secondary systems controlled by writing service GABA, so that the output of excitatory neurotransmitters tends to be restored, and/or the sensitivity of their receptors increases. The whole complex of primary and secondary changes eventually results in benzodiazepine tolerance. This pharmacodynamic tolerance develops unevenly to different benzodiazepine effects. For example, tolerance appears more rapidly to hypnotic and anticonvulsant than to anxiolytic effects (Sepinwall Cook, 1979). Tolerance to Iago's different effects may also vary between individuals, possibly due to variations in writing intrinsic GABA activity in Essay on The Sexuality Of Women In Beowulf: vs. Print different parts of the brain, which are in turn reflected in personality characteristics and susceptibility to stress. Tolerance is never complete and probably never reaches a perfect equilibrium in all brain systems, which may be one reason for the high morbidity amongst chronic benzodiazepine users (Ashton, 1987). Acute tolerance, especially to hypnotic effects, can be manifested rapidly, but chronic tolerance develops over thesis writing service, a time-course of several weeks. Once established, chronic tolerance can last for months or even years after cessation of Interview With Resources, some central nervous system depressants such as alcohol (Cicero, 1979) and probably also benzodiazepines. The development of pharmacodynamic tolerance sets the scene for thesis writing service, the withdrawal syndrome.

Cessation of the drug exposes all the adaptations which have accrued to counteract its presence, releasing a rebound of unopposed activity involving many neurotransmitters and their receptors and many brain systems. Clinically this state is manifested as the withdrawal syndrome, consisting of effects that are largely the opposite of those originally induced by the drug. The distribution, duration, and severity of symptoms depend on the particular systems that have undergone adaptive modulations and the degree of the adaptive changes induced, as well as on the rate of drug withdrawal. Some authors distinguish between rebound and creole man withdrawal effects, but the mechanism is the same for both (Lader, 1988) Acute withdrawal effects are reversed by an appropriate dose of the drug, which restores the status quo . As the homeostatic changes slowly reverse, withdrawal symptoms decline. The process of reversal, like that of tolerance acquisition, does not necessarily proceed evenly in thesis service all systems. The variable time of emergence and duration of individual symptoms during benzodiazepine withdrawal noted by Civil Tyrer (1989) and Ashton et al . (1990) (Table 1) may reflect this uneven course.

The perturbations of thesis writing service, brain function induced by benzodiazepines are exceedingly complex, and it is Soliloquy Analysis Essay not surprising that withdrawal symptoms are many and variable. Different symptoms may reflect disturbance of the balance between different neurotransmitter systems as suggested by Ashton (1984) and are likely to show large interindividual differences depending on thesis writing service personal characteristics and susceptibilities. As discussed above, it is difficult to Essay Sexuality set a definite time limit on the reversal of tolerance and, therefore, the end of the thesis writing, withdrawal syndrome. Of Hot Water Essay! In general, tolerance declines over a matter of weeks, but in some cases it may endure for a year or more (Cicero, 1979). Delayed or slow reversal of tolerance may account for thesis writing, some protracted withdrawal symptoms. However, some changes induced by Essay on The Of Women In Beowulf: Film benzodiazepines may be permanent or only very slowly reversible. Thesis Service! Since benzodiazepines apparently inhibit learning, especially for coping with stress strategies (Gray, 1987), cessation after many years of between, use may expose a learning deficit, especially in the ability to cope with stress. Thesis Service! This may persist as protracted anxiety, and may possibly be related to protracted depression.

Anxiety symptoms are likely to endure until new learning has induced the appropriate synaptic changes, which probably involve modification of Civil War Started? Essay, endogenous GABA activity. Finally, there remains the service, question of whether benzodiazepines can cause structural neurological damage. Like alcohol, benzodiazepines are lipid soluble, are highly concentrated in the brain, and impair cerebral cortical, cerebellar, and limbic system function. It is possible that use over many years could cause physical changes such as cortical shrinkage, which may be only creole man, partially reversible. Such changes have been demonstrated by CAT-scan studies in young alcoholics (Lee, Möller, Hardt, Haubek, Jenson, 1979; staff, British Medical Journal, 1981) although not conclusively in chronic benzodiazepine users (Lader Petursson, 1984; Perera, Powell, Jenner, 1987). Thesis Writing! However, such techniques may not be sensitive enough to detect subtle changes. Nor are standard tests of a Human Resources, intellectual function sensitive enough to detect minor degrees of cognitive impairment that may persist after withdrawal in some long-term users. It remains possible that some protracted benzodiazepine withdrawal symptoms (including tinnitus and thesis service other neurological and psychological symptoms) could result from physicochemical neuronal damage. These symptoms would not be fully relieved by restarting benzodiazepines.

There are still many puzzling features of benzodiazepine withdrawal, and the benzodiazepine story remains unfinished (Ashton, 1984). Ashton, H. (1989). Risks of dependence on benzodiazepine drugs: A major problem of uk, long-term treatment. British Medical Journal , 298, 103-104. Ashton, H., Golding. J.F. (1989). Tranquillisers: Prevalence, predictors and possible consequences.

Data from a large United Kingdom Survey. British Journal of Addiction , 84,541-546. Ashton, H., Rawlins, M.D. Tyrer, S.P. (1990). Thesis Writing Service! A double blind placebo controlled study of Essay Interview With Resources, buspirone in diazepam withdrawal in chronic benzodiazepine users. British Journal of Addiction, 157, 232-238. Ashton, H., Stepney, R. Thesis Service! (1982) Smoking: Psychology and pharmacology , London: Tavistock Publications. Benton, D., Rick, J.T. Difference Between! (1976). The effect of increased brain GABA produced by amino-oxyacetic acid on thesis writing service arousal in rats.

Psychopharmacology , 49, 85-89. Bloom, F.E. (1985). Neurohumoral transmission in the central nervous system. In A.G. Gilman, L.S. Goodman, T.W. Rall, F. Muland (Eds.), The pharmacological basis of therapeutics (pp. 236-259). New York: Macmillan. Busto, U., Fornazzari, L., Naranjo, C.A. Creole Man! (1988).

Protracted tinnitus after discontinuation of service, long-term therapeutic use of The Benefits, benzodiazepines. Journal of Clinical Psychopharmacology , 8, 359-362. Busto, U., Sellers, E.M., Naranjo, C.A. Cappell, H.P., Sanchez, C.M., Sykora, K. (1986). Withdrawal reaction after long-term therapeutic use of benzodiazepines. New England Journal of Medicine , 315, 654-659. Cicero, T.J. (1979). Tolerance to and physical dependence on alcohol: Behavioural and neurobiological mechanisms. in Brain and pituitary peptides (pp.1603-1617).

Munich: Ferring Symposium 1979. Costa, E. (1981). Thesis Writing Service! The role of gamma-aminobutyric acid in the action of 1,4 benzodiazepines. In J.W. Lamble (Ed), Towards understanding receptors (pp. 176-183). Amsterdam: Elsevier/North Holland.

Cowen, P.J., Nutt, D.J. (1982). Abstinence symptoms after withdrawal from tranquillising drugs: Is there a common neurochemical mechanism? Lancet ,2,360-362. Gilmartin, J.J., Corris, P.A., Stone, T.N., Veale, D., Gibson, G.J. (1988). Effects of diazepam and chlormethiazole on ventilatory control in normal subject.

British Journal of Clinical Pharmacology , 25, 766-770. Golding, J.F., Cornish, A.M. (1987). Personality and life-style in medical students: Psychopharmacological aspects. Psychology and Health , 1, 287-301. Golding, J.F., Harper, T., Brent-Smith, H. (1983). Personality, drinking and drug-taking correlates of cigarette smoking. Personality and Individual Differences , 4, 703-706. Gray, J.A. (1981).

Anxiety as a paradigm case of emotion. British Medical Bulletin , 37, 193-197. Gray, J. A. (1987). The neuropsychology of emotion and The Benefits of Hot personality. Thesis Writing Service! In S.M. Stahl, S.D. Iverson, E.C. Goodman (Eds.), Cognitive neurochemistry (pp.171-190).

Oxford: Oxford University Press. Haefley, W., Pieri, L., Pole, P., Schaffer, R. (1981). General pharmacology and neuropharmacology of benzodiazepine derivatives. In H. Hoffmeister, and G. Stille (Eds.), Handbook of experimental pharmacology (Vol. 55, 11, pp. 13-262). Between Uk! Berlin: Springer-Verlag. Hallström, C., Lader, M. Thesis! (1981). Benzodiazepine withdrawal phenomena. Creole Man! International Pharmacopsychiatry , 16, 235-244. Hermann, J.B., Brotman, A.W., Rosenbaum, J.F. Thesis Writing Service! (1987).

Rebound anxiety in panic disorder patients treated with shorter-acting benzodiazepines. Journal of Essay With a Human, Clinical Psychiatry , 48 (suppl. 10), 22-28. Kales, A., Scharf, M.B., Kales, J.D. (1978). Writing! Rebound insomnia: A new clinical syndrome. Science , 201, 1039-1041. Lader, M. (1988). The psychopharmacology of addiction - Benzodiazepine tolerance and dependence. In M. Lader (Ed.), T he psychopharmacology of addiction (pp. 1-14). Oxford: Oxford University Press.

Lader, M.H., Olajide, D. (1987). A comparison of buspirone and placebo in relieving benzodiazepine withdrawal symptoms. Journal of Clinical Psychopharmacology , 7, 11-15. Lader, M.H., Petursson, H. (1981). Benzodiazepine derivatives - Side effects and dangers.

Biological Psychiatry , 16, 1195-1212. Lader, M.H., Ron, M., Petursson, R.H. (1984). Computed axial brain tomography in long-term benzodiazepine users. Psychological Medicine , 14, 203-206. Lee, K., Moller, L., Hardt, F., Haubek, A., Jenson, E. (1979).

Alcohol-injured brain damage and liver damage in War Started? Essay young males. Writing Service! Lancet 2, 759-761. Lum, L.C., (1987). Hyperventilation syndromes in medicine and psychiatry: A review. Journal of the Royal Society of Medicine , 80, 229-231. Möhler, H., Okada, T. (1977). Benzodiazepine receptors: Demonstration in on The the central nervous system.

Science 198, 849-851. Murphy, S.M., Owen, R.T., Tyrer, P.J., (1984). Withdrawal symptoms after six weeks treatment with diazepam. Thesis! Lancet , 1389. Murphy, S.M., Tyrer, P. Creole Man! (1988). The essence of benzodiazepine dependence. (pp. 157-167).

Oxford: Oxford University Press. Nutt, D. (1986). Benzodiazepine dependence in the clinic: Reason for anxiety? Trends in Pharmacological Sciences 7, 457-460. Olijade, D., Lader, M. (1984). Depression following withdrawal from long-term benzodiazepine use: A report of writing service, four cases.

Psychological Medicine , 14, 937-940. Oswald, I. (1989) Triazolam syndrome 10 years on. Sexuality Of Women In Beowulf: Vs. Print! Lancet 1, 451-452. Owen, R.T., Tyrer, P. (1983) Benzodiazepine dependence: A review of the evidence. Drugs , 25, 385-398. Perera, K.M.H., Powell, T., Jenner, F.A. (1987). Computerised axial tomographic studies following long-term use of benzodiazepines. Writing! Psychological Medicine , 17, 775-777. Petursson, H., Lader, M.H. Of Hot! (1981a) Withdrawal from long-term benzodiazepine treatment.

British Medical Journal , 283, 634-635. Petursson, H., Lader, M.H. (1981b). Thesis Writing! Benzodiazepine dependence. British Journal of Addiction , 76, 133-145. Rementeria, J.L., Bhatt, K. (1977). Withdrawal symptoms in Why The Civil War Started? Essay neonates from intrauterine exposure to diazepam. Writing! Journal of Paediatrics , 90, 123-125.

Sepinwall, J., Cook, L. Creole Man! (1979). Writing! Mechanisms of difference undergraduate, action of the benzodiazepines: Behavioural aspects. Writing Service! Federation Proceedings 39, 3024-3031. Smith, D.E., Wesson, D.R. Iago's Analysis! (1983). Thesis Service! Benzodiazepine dependency syndromes. Journal of Psychoactive Drugs , 15, 85-95. Squires, R.F., Braestrup, C. (1977). Benzodiazepine receptors in the rat brain.

Nature , 266,732-734. Staff. (1981). Minor brain damage and alcoholism. British Medical Journal , 2, 455-456. Tyrer, P. (1990) Diagnosis of benzodiazepine dependence . Unpublished manuscript.

Tyrer, P., Murphy, S., Riley, P. Interview With A Human! (1989). The benzodiazepine withdrawal symptom questionnaire. Journal of writing service, Affective Disorders , 19, 53-61. Tyrer, P., Owen, R., Dawling, S. (1983). Gradual withdrawal of diazepam after long-term therapy. Lancet 1, 1402-1406.

Tyrer, P., Rutherford, D., Higgett, T. (1981). Benzodiazepine withdrawal symptoms and of Hot propranolol. Lancet , 1, 520-522. Winokur, A., Rickels, K., Greenblatt, D.J., Schatz, N.J. Thesis Writing Service! (1980). Between Undergraduate And Postgraduate! Withdrawal reaction from long-term low dosage administration of diazepam. Archives of General Psychiatry , 37, 101-105.

Young, W.S., Kuhar, M.J. Thesis Writing Service! (1980). Radiohistochemical localisation of Why The Civil War Started?, benzodiazepine receptors in rat brain. Thesis! Journal of Pharmacology and Iago's Soliloquy Experimental Therapeutics , 212, 337-346. Zigmond, A.S., Snaith, R.P. (1983). The hospital anxiety and depression (HAD) scale. Acta Psychiatrica Scandinavica , 67, 361-370.

FIGURE 1. WITHDRAWAL SYMPTOMS RATING SCALE. Ratings: 0=none 1=mild 2=moderate 3=severe. FIGURE 1. Withdrawal Symptoms Rating Scale. FIGURE 2. Writing! Hospital Anxiety Depression (HAD) Scale scores for anxiety over 20 weeks in War Started? 18 healthy students and 12 patients taking diazepam (7.5 SD 4.6 daily). Patients and students took placebo tablets until week 20. Patients withdrew from diazepam during weeks 8-12. One patient dropped out service, after 12 weeks for domestic reasons. Circles represent placebo group; triangles represent students.

FIGURE 3. Withdrawal symptoms scores over 20 weeks in 18 healthy students and The Benefits Water 12 patients taking diazepam (7.5mg SD 4.6 daily). Patients and procedures as in Figure 2. Symptom rating shown in Figure 1. Circles represent placebo group; triangles represent students. TABLE 1. Scores for selected symptoms before, during, and after diazepam withdrawal. ( n = 11; mean total scores on withdrawal symptoms scale shown in Figure 1. (postwithdrawal score equal. to or less than prewithdrawal. greater than prewithdrawal. Sensory hypersensitivity: to light, sound, taste, smell.

Anxiety: Hospital Anxiety Depression (HAD) Scale (anxiety) scores. Paraesthesiae: tingling, numbness, altered sensitivity in limbs, face, trunk. Motor symptoms: total score for writing, stiffness, weakness, tremor, muscle twitches, ataxia.

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Not including it increases the chances that your resume will be removed from thesis writing service, consideration. Creole Man! This is because the information is often required for the resume to move through the process and it isnt easily attainable by the staffing office. So, theyll often pass on a resume that doesnt include the information in favor one that does. Provide at least the following information about your education: Name of thesis writing service school Degree earned Beginning date Completion date City, and creole man State. You might also consider adding a description that includes achievements, awards, scholarly organizations and activities, or your GPA if it was really high.

Of course, scholastic achievements are more important the earlier you are in your career, so keep that in mind when crafting the education section of writing your resume. Finally, you may also wish to include details on any continuing education units youve taken within the Civil, last 2 years. Bonus: Are you bilingual? If so, be sure to include it on your resume! According to a recent study from Wanted Analytics, bilingual was the writing service, second most commonly required skill listed on nursing job advertisements. 7. Nursing License and The Benefits Water Heaters Certification Details: It is writing service highly recommended that you include the following for your licenses: License type (LPN, RN, NP, CRNA, etc.) Licensing State/Body Name on license if different from creole man, name on resume License expiration date License number If the license is part of the service, Nurse Licensure Compact, then state it clearly.

Many people argue that a nursing license number should not be included on creole man, a resume as a matter of privacy. Writing Service! However, nursing license numbers are a matter of public record. They can be easily obtained from each states Board of Nursing using the standard information included on your resume. Providing the license number simply makes the recruiters job easier. And in of Hot Water a competitive job market, that could make all the difference in thesis the world. When it comes to creole man certifications, it is highly recommended that you include the following for every certification you hold: Certification name (BLS, ACLS, PALS, TNCC, etc.) Certifying body (AHA, etc) Expiration date, or date acquired if it has no official expiration date. Unlike most professions, nursing is conducted round-the-clock. Therefore, its best to convey the shifts youre willing to work on your nursing resume.

Are you open to working 8, 10, and/or 12 hour shifts? Are you open to thesis service working Days, Mids, PMs, and/or Nocs? You should include this information even if youre applying for a specific job with a specific shift. You never know if the employer has another opening that is unadvertised which you maybe qualified for. Moreover, resumes arent always submitted to specific job advertisements. In fact, many sources indicate that 80% of all jobs are filled through networking. Why The! Including your availability is very helpful for general job inquiries. In addition, you should indicate your willingness to relocate when applicable.

These statements regarding availability can be given their own heading, but its best to service simply add them to your Professional Summary. The healthcare industry is Iago's Soliloquy Essay going electronic. Paper charting will soon be a way of the past. Its imperative to list any and all Electronic Health Record (EHR) and Electronic Medical Record (EMR) experience you have. According to a recent study by Wanted Analytics, Electronic Medical Record was the most commonly required skill for nurses listed in nursing job advertisements. Epic Software and Meditech, popular EMR software packages, were also on the list. Of course, billing codes are a big part of EMRs. And both ICD10 and writing service ICD 9 are among the The Benefits Heaters, most commonly listed skills in job advertisements.

So if your scope of practice deals with this in any way, then be sure to add these to your resume. You may also want to include any other computer experience you have just in thesis case it may be of value to the employer, or to at least demonstrate that you have computer skills if you lack experience with EHRs and EMRs. Signifying the Essay Sexuality Of Women vs. Print, type of thesis writing facility you worked in tells the potential employer a ton about your experience with very few words. You should know the exact designation of Essay about Interview With a Human Resources all the facilities you worked with and writing service if you dont, now is the time to find out. Short term acute care? Long Term Care? Long Term Acute Care? Senior Nursing Facility? In addition, if you worked at a Trauma Hospital, then you should include this on your resume along with the trauma designation (1, 2, 3, etc).

If you worked at Soliloquy a teaching hospital, then you should include this information. Such information can be included under the specific job description in your resumes Work History section, or in thesis writing your resumes Summary. For example, if the job listing youre applying for lists trauma hospital experience as necessary or desired, then its a good idea to identify your trauma experience in the resume Summary to make it stand out. Include the number of total beds at the facilities you worked at, as well as the number of beds in the specific units on Essay about Interview With Manger, which you worked. Just like the thesis service, facility type, providing the number of beds tells the potential employer a ton about your experience with very few words. We regularly saw resumes that listed things like 3 West as the unit. Essay Film Vs. Print! Unfortunately, only people who work at thesis writing that facility know what that means.

Instead, include the type of between unit (MS, TELE, ICU, CVICU, ER, LD, etc) in order to convey the thesis writing, message. We cant stress enough how important this is. Also include your caseload. What was the about With, nurse to thesis patient ratio in this unit, and how much support was there in the form of LPNs, CNAs, or MAs? Include any other unit specific details as well. Did the unit take trauma patients? Did the unit routinely deal with overflow from other units?

If so, what types of patients were seen from overflow? 1. Specific Details/Duties and Heaters Essay Accomplishments Regarding Your Nursing Experience: Up to this point, the focus has been on incidental, although very important, details. But what about the meat of your resume, the thesis service, job descriptions and experiences that make up the Essay on The Of Women In Beowulf:, bulk of your resume? When it comes to writing service this, nurses are in the precarious position of balancing duties with accomplishments. You see, the current standard recommendation for resumes is to make them accomplishment driven. The idea is Essay on The Sexuality that you should provide specific accomplishments, as opposed to thesis writing service duties, that illustrate how your work translated into quantifiable and tangible results for your current and Water previous employers. Thesis Writing Service! Advocates of creole man this approach advise against listing duties on your resume.

However, healthcare employers need to know that you have experience with the highly technical duties that are integral to the job youre applying for. Writing Service! At the same time, they too want to know about your accomplishments. Civil Essay! Therefore, its important to include details about the thesis writing, specific day-to-day duties you performed. However, at the same time, you dont want to fall into the trap of of Hot Heaters creating a duties-driven resume. Managing this challenge represents a key difference between nursing resumes and general resumes. Before we continue, its best to address the 800 pound gorilla in this conversation. Thesis! Nurses sometimes have hundreds of Soliloquy Essay duties and responsibilities. How do you choose which ones to list because you certainly cant list them all?

We hate to say it, but including things like, Provided patient care is a complete waste of space. Its simply too general. Our experience indicates that many nurses believe that nursing is very similar no matter where you go which may be the reason that such general statements are so often included in nursing resumes. Thesis Writing Service! In reality, the differences are stark between various facilities. For example, the Step-Down Unit at one hospital may not work with Swan-Ganz Catheters while the SDU in another hospital may. With that in Civil mind, here are some general themes to consider when determining the types of duties to thesis writing service include on your resume. This list is not comprehensive, but it should help you understand the level of specificity that were talking about:

Did you start IVs? Did you administer medications? Which medications? What type of patients did you care for? Renal? Cardiovascular? Neuro? Ortho? Rehab? What was the age range of the patient population you cared for? What specific equipment do you have experience with?

Did you read strips? Work with vents? Trachs? Balloon pumps? Swan-Ganz? da Vinci Surgical System? Which industry-wide protocols, processes and Iago's Soliloquy procedures are you experienced with? For example, AIDET is writing among the most commonly listed requirements in nursing job advertisements. There are many similar protocols, processes and procedures throughout the industry, so be sure to make note of those that were used by Analysis Essay, your previous employers.

Now, were still in the tough spot of determining exactly which skills and duties to include on the resume. First, start by including any duties specifically mentioned in thesis writing the job description that you have experience with. Next, do some research on the employer in Civil question to find specific details that may help you decide which duties might be important to list. Use the company website, news, and any professional connections you have in an effort to determine the thesis, types of patients, processes and procedures common to the specific employer in Essay about Interview Resources question. Once you have the duties narrowed down, there are a couple of ways to convey them on thesis service, your nursing resume. First, you can incorporate the duties into a Human Resources Manger your accomplishment statements. We discuss this approach below. Second, you can simply list out the duties. Writing! While this is less preferred, it is sometimes not possible to accomplish any other way. Of Women In Beowulf: Vs. Print! Your summary is a good place for this. For example, you might include the writing, following in your resume summary if youre applying for a position in the CVICU:

Proficient with starting IVs, Intra-aortic balloon pumps, 12-Lead Placements, 12-Lead Interpretation, AICD Insertion, Beta Blockers, Argotroban, Atropine and other cardiovascular medications. Bonus: Given the large number of Why The Civil War Started? skills and writing service duties nurses are responsible for, you may consider utilizing a Skills Checklist during your job search. Skills Checklists are self assessment tools commonly used throughout the healthcare industry. BluePipes provides members with free access to over 100 comprehensive skills checklists that can be completed, saved and downloaded as PDF documents. You can view a sample here. Theyre a great way to convey your skills to potential employers. You might consider uploading them along with your resume when applying for jobs or you can bring them to Iago's Soliloquy Essay your job interviews. Considerations for Accomplishments. Now that weve covered duties, lets take a look at accomplishments. First, you can try to thesis writing service frame duties inside accomplishment statements in creole man order to knock them both out at the same time.

In other words, offer an explanation about how you achieved results while performing your duties. Again, this isnt always possible, but here are some considerations: Did you receive recognition or awards from your previous/current employer? Did your previous/current employer receive recognition or rewards? How did performing your duties contribute to thesis service that?

When framing accomplishments, its also useful to creole man know how your previous and current employers quantified their success as an organization. For example, patient satisfaction is typically tracked with programs like HCAHPS or Press Ganey. Did your employer experience improvements with such indicators? How did performing your duties influence that? Next, its important to understand how your current and thesis writing service former employers measured your individual performance as this is very helpful when framing accomplishments. Nearly every healthcare employer conducts employee evaluations. Evaluations typically offer both qualitative and quantitative information that can be leveraged when framing accomplishment statements.

Again, you may choose to list specific duties youre proficient with separate from your accomplishments. Or, you may choose to frame the duties within your accomplishment statements. Or you may choose a combination of the two approaches. In any case, its important to provide both job-specific duties and accomplishments on your nursing resume. Bonus: Healthcare is very diverse. Many hiring managers would also like to know what other skill sets you have outside of Interview With Resources your primary area of expertise. Writing! Did you float to the ER or LD units when needed?

If so, then you may want to provide some reference to the skills and between undergraduate and postgraduate accomplishments you achieved in thesis service these areas. General perspective on nursing resumes. This may seem like a ton of information to incorporate into Why The Civil a standard resume. However, nursing is not a standard profession and thesis concerns over resume length are becoming antiquated with the advent of Iago's Essay Applicant Tracking Systems. Moreover, the push to thesis force nursing and Interview a Human Resources Manger healthcare resumes to thesis writing service conform to Soliloquy Analysis the standard format that serves general professionals, like salespeople, is writing a disservice to Why The War Started? Essay both healthcare professionals and employers. Healthcare professionals often miss opportunities to highlight skills and experience that are highly sought after. As a result, healthcare employers often miss out on perfect candidates. This push toward generalized conformity is thesis service even prevalent on the most popular job boards, like Monster and CareerBuilder, and professional networking services like LinkedIn.

Nurses and other healthcare professionals are better served by industry specific professional networking services like BluePipes. For example, BluePipes provides nurses with a profile builder capable of difference between uk recording healthcare specific career details as well as the ability to print the profile to PDF as a resume formatted specifically for thesis writing, healthcare professionals. So its like a cloud based resume service. It also offers several other tools that help nurses manage their careers more effectively and efficiently. As always, your feedback is greatly appreciated.

Please let us know what you think by posting a comment! 16 Tips To Prepare Your Nursing Resume For Applicant Tracking Systems Weve spoken to countless nurses and other healthcare professionals who. How to Write the Best Nursing Cover Letter A well crafted nursing cover letter can be just as. Nursing Resume Objective or Summary? Nurses often wonder if they should include an objective or. I am wondering how to list my experience as a maternal-child float RN of Civil War Started? 2+ years. I worked 4 different units, all quite different, ranging from mom baby, to peds, peds ed/uc, and nicu. How do i go about addressing those different areas and responsibilities and thesis writing service skills, when they all fell under 1 position? Thank you for the information, its very helpful. This site is of so much help to me. thank you, and it is The Benefits of Hot Water Heaters Essay also free.

Its amazing, I just cant appreciate it enough. I was wondering, i am a one year experience nurse at a step down ICU (PCU), I am now looking for a job in an ICU. The locations and facility i am looking at have nothing for experience nurses, but have a lot of thesis writing positions for new nurses that want to work in ICU. I just want to apply to the new nurse position and i really dont mind the pay. Now my question is, should i just use my new grad resume with my clinical experience or i should update my resume with my one year experience and apply although the position is for new grads. Undergraduate And Postgraduate! please i will really appreciate any advise. thanks you. How can I include my Medical-Surgical Certification from ANCC on my resume. Thesis Writing Service! Can I include it on my credentials area on top of my resume following my name and creole man degree? I ask because the ANCC instructs to do so on their website.

Ill be honest its somewhat confusing though, at least to me. I plan to thesis include my Medsurg certification in certification section of my resume where BLS and RN licensure are, etc. I just wanted it to stand out and Essay In Beowulf: pop so to speak as to thesis service not have to read on to notice it. Creole Man! On ANCC site, on a How to Display Your Credentials page they instruct you to thesis service include RN- BC (Registered Nurse-Board Certified). Was hoping you could clarify or add something else. Great web site thanks.

Thanks for on The Of Women, the inquiry, Jesse, and my sincerest apologies for the delay! Your Medical-Surgical Certification from ANCC is thesis service considered a National Certification and should be listed after your name at Analysis the top of your resume and in the Licenses and Certifications section of your resume. The order for listing credential after your name is: Highest Degree Earned State License National Certification Nationally Recognized Honors and Awards. I hope this helps! I have been working out writing of the acute care setting in public health nursing for Essay, 7 years. I have heard over writing service, and over that because I have been out creole man of the acute care setting for so long that I dont qualify for a lot of the thesis service, positions that I have applied for. Essay About Interview A Human Resources! Admittedly, I have not used many nursing skills for the last 7 years, except for giving immunizations and occasionally drawing blood. Service! I have gotten very weary and and postgraduate feel like I will not be able to find another job, and I am really not happy with my current job. Writing! I am unsure of The Benefits Heaters Essay how to thesis make my resume pop and between have prospective employers want to give me a chance.

I am not looking to get back into areas that are highly skilled, but I would not mind getting back into acute care. Any ideas. Kurt, I am an RN at a VA hospital in cardiac telemetry. One of the biggest hurdles to applying at any VA facility is the fact that USA Jobs is thesis service a digital screening tool as much as it is an online application portal. Matching as many keywords in the functional statements for your desired role in your application can be the difference between getting an interview and being passed over.

Hope this helps! In your article you talked about whether or not your previous/current employer received recognition or rewards. Im working on creole man, applying for a position that is open for an orthopedic nurse after quitting my previous orthopedic nurse job. The hospital that I previously worked received recognition for their total joint replacement program. I was wondering if this would be something that is good to include as part of my resume, considering I was a part of the unit during that time for the hospitals certification/recognition. If so, how would you go about including that in without making it seem just a random fact listed and make it more as an accomplishment for thesis, myself also since I was a part of that unit/team during that time. Thanks for the inquiry, Jessica. Yes, this is definitely something you can include on your resume. Civil War Started?! If you feel that youre not able to expand in a way that adequately describes your role in the achievement, then you may want to expand in your cover letter. On your resume, you might want to couple this team achievement with one of your own that demonstrates you were a key contributor. For example, you might have received an individual award or a high employee evaluation score.

You may also want to include this in your summary as opposed to thesis writing the job description to creole man make it stand out a little more. I hope this helps! I am an experienced OR nurse who has performed in just about every surgery setting from Open heart to Ophthalmology, outpatient as well as pre-op admitting and recovery In the past I have managed a surgery department at thesis writing a busy hospital and also ran a surgery center. I have been fortunate to have been able to take off time to Essay on The Of Women Film vs. Print stay home and be with my children. Service! That being said, I am worried about the years off and blank space on my resume. How would you go about filling in The Benefits of Hot Heaters Essay the gap? I have volunteered in multiple areas at their schools, from organizing an Emergency Preparedness fair, fundraisers and teaching Compression Only CPR. My license and continuing CEUs have always rained current. Do you think this will make it difficult for me as I try entering the job setting? I appreciate any suggestions you might have. Thanks for the inquiry.

There is no steadfast rule on how to handle this situation, but there are two fundamental approaches to consider. First, you can consider a Functional Resume. Functional resumes are resumes that focus on skills instead of experiences. In your case, you would focus on all of thesis writing your OR experience. You might have a small section to list your previous employers. or you may even choose to leave it out entirely. There are many examples of Functional Resumes available on the internet. Second, you could use a traditional chronological resume. In this case, you would list your experience raising the War Started? Essay, kids as one of the entries in your chronological work history. You could include details on the experiences mentioned in your comment on this blog post. Again, there is no right or wrong way.

Each has its advantages and disadvantages. To answer your question, yes, this situation is always a bit of challenge. However, experienced nurses are in service high demand at and postgraduate uk the moment, so you should be fine. Also, Im personally inclined to recommend the chronological format. The main advantage of Functional Resumes in thesis writing this particular case is to draw attention to your skills and Iago's away from the thesis writing service, gap in recent experience. However, employers are going to find the gap no matter what. So, I think its best to embrace it.

With the chronological resume, you can still include all the applicable skills. I hope this helps! I have worked in home health and corrections for about 5 years now. I have done a lot of basic nursing ranging from blood draws, interpreting labs, starting IVs, wound care, peritoneal dialysis, picc line care (including using clot busters) and removal. IV anti biotics, med passes small and Iago's Analysis Essay large including mental health medication passes. I have responded to chest pain calls, shortness of breath, hangings, falls, knife wounds, self inflicted wounds, and unresponsive patients. I have worked in a corrections hospital with acute and long term patients as well as hospice patients, but it isnt seen as ER work even though it seems from talking to other nurses that that isnt so far different than those working in a hospital ER.. Trying to thesis service format my resume to difference and postgraduate uk make my experience fit into service what the Veterans Administration would want/be attracted to.. I am a soon to be retiring Military Nurse and will be transitioning to the civilian workforce. How important are listing awards?

I have numerous military awards based on my work ethics and difference between undergraduate and postgraduate performance on the job, but I am afraid that the general public is thesis writing service not going to The Benefits Essay have any idea what they are or mean. Writing Service! Also, any good tips on turning military missions into civilian language? Thanks for difference undergraduate uk, your service!! I think its a great idea to add the thesis writing service, awards to your resume. You could do one of two things. Simply list them out by their official name. Creole Man! Theres a good chance that the reviewer will search for the award on thesis, the internet and find it.

Or, you could add a very brief description of the award in parentheses. For example: X Award (earned for valor in action). Either way, if you have a lot of awards, then you may want to include only the on The Sexuality Film vs. Print, highest ranking awards. If you choose to thesis list many of them, then put them in columns or in Essay a continuous stream separated by thesis, commas to save space. Conveying your military experience in civilian language can be challenging if you did not work in a military hospital. My apologies, but I lack the technical expertise to provide detailed recommendations. War Started?! That said, Id recommend reviewing the job descriptions for the jobs youre applying for use them to guide your efforts whenever possible. I hope this helps and thesis thanks again! I have a position working in a float pool (we call it the Mobile Unit). The unit is split into two different tracks, med/surg (HV general acuity patients; ortho/urology/gen/gyn surgery; neuro/ENT; Onc [recent unit merge means I dont float here anymore]; gen med; adult psych; child/adolescent psych; Postpartum (moms and babies); inpatient rehab) and critical care track (Onc/Med/Surg IMCU; ICU; HV high acuity patients; ER; Neonatal ICU).

How do I discuss bed numbers for The Benefits Water, each unit and writing service descriptions that highlight any specific training I have had to play into each patient population? It seems like theres no way for me to about Resources Manger cover all of this detail without leaving a lot out or getting too wordy. I also accepted a critical care position, but have not transitioned yet. My husband just got a job out of state, so we have to relocate, as much as I love my current employer. This is a great question; thanks for posting it here!

Youre right, youre probably not going to be able to writing convey the broad spectrum of your experience without getting too wordy for your nursing resume. So, youll need to on The Film offer some general details as well as some specific details. When it comes to thesis writing specifics, its best to tailor your resume to the specific job youre applying for. Essay On The Of Women In Beowulf: Vs. Print! Find out what theyre looking for and be sure to convey that you have the skill set, or convey relevant details about your experience. I believe this is the most important consideration for your resume. When it comes to generalizations, you could offer a description similar to the one youve provided here. For example: I worked on thesis writing service, a Mobile Unit, or Float Pool, caring for Med/Surg patients 50% of the time and ICU patients 50% of the time. The Med/Surg patients included Postpartum, PSYCH, general Med/Surg, and Neuro/ENT.

The ICU patients included ER, NICU and general ICU. Unit sizes varied from 5 beds up to 25 beds. You might also try utilizing skills checklists to difference between undergraduate uk convey your experience, especially if you make it to the interview stage. Also, many applicant tracking systems allow applicants to upload documents, so you might be able to upload skills checklists there. You can complete and save skills checklists on BluePipes and utilize them at writing service your convenience. I hope this information helps! My husband was an ICU nurse for 5 years (18 months of that he was an ICU travel nurse) but took 2.5 years off to work in another field. Hes now applying for nursing positions as we prepare to move.

Should he address the clinical gap in his resume? With just his last RN job listed, it looks like he last worked in 2013 though hes been employed as a youth minister since then. How should he handle this? Yes, you should address the gap in the resume. Its fairly common for hospitals to require the last 7 years of work history to be included on their job applications. A large percentage of the hospitals I worked with had similar requirements for resumes. Unfortunately, the default assumptions when it comes to Why The Civil Essay employment gaps are all negative.

Do your best to thesis writing service tie the Sexuality Of Women vs. Print, experience into nursing. I believe most career advisers would recommend the service, same. I hope this information helps! I am applying for RN jobs, but am still waiting to take my boards (will take them within the next 1-2 months). How should I address this on my resume? You may want to add an excerpt under Licenses and Certifications or your Summary (or wherever appropriate) that provides the date youre scheduled to take the exam. For example: RN: Scheduled for NCLEX on July 9, 2015.

I hope this helps! Thank you! I think thats what ill do. For a new graduate of a second-degree BSN program, would you recommend including GPA (3.9) on a resume? Thanks for the question, Emily! Yes, I recommend adding a great GPA to your nursing resume. We discuss this in our blog post on new grad resumes and in our blog blog post on job search tips nurses should avoid. Many people argue that your GPA doesnt matter. They say that all that matters is that youre licensed.

If that were the case, then no details about you as a person, your work ethic, or achievements would matter either. And we know thats not true. It is HIGHLY unlikely that your resume is passed over because you included your high GPA. Creole Man! Meanwhile, many hospitals and hiring managers love to see it, and assign value to it. So yes, by thesis, all means, add it. The Benefits Of Hot Essay! I hope this helps!

Great work, by service, the way! I am wondering if I should include phone numbers for Interview, my previous employers? If yes, which number should I use the thesis writing service, general number, the unit, or HR? Also, some of creole man my employment history goes back many years and the identifying information(number of beds, etc.) has changed. I do not have the correct information from when I worked there. How should I list this information? Is there a good way to find current identifying information for a hospital? Thanks in advance for your response.

Thanks for posting these great questions! First, there are no set rules for writing service, nursing resumes, so youll find some disagreement on almost every resume recommendation. That said, the general rule is that you should not include the The Benefits, contact telephone numbers for your previous employers on your resume. In fact, you dont need to writing include the complete address either. The city and state will suffice for your resume. On a side not, chances are that youll be applying for most jobs on the companys website through an applicant tracking system.

These online applications may allow you to Iago's Soliloquy Analysis Essay enter the telephone numbers and addresses for your former employers. In this case, I always recommend adding every last bit of information you can to your online applications. Again though, thats separate from thesis service, your resume. On a another side note: If you are applying for travel nursing jobs, then you should include the telephone numbers and the supervisor names for your previous jobs. In order to creole man find current information for your former employers, you can use a website like The American Hospital Directory. They have a free hospital profile lookup tool. I recommend using the advanced search for best results. Please note that the links to these pages are underlined in blue. Here you will find the current contact information, number of beds, teaching hospital status, trauma status, etc. If you are unable to thesis writing service locate the creole man, information here or if your former employers are not hospitals, then you can simply try a google search for them or try the Medicare.gov site. If your former employer does business with Medicare, then they should be in the database with current informationassuming they want to get paid :-).

Now, about your older work history. Many resume experts recommend including only the last 10 years of work history on your resume. However, that assumes that your prior experience may no longer be applicable to your current job search. Thesis! Others argue that you shouldnt include more than 10 years of Essay about Interview a Human Resources Manger work history because you dont want to date yourself. Theyre concerned about ageism in the hiring process.

These same people recommend not to include the dates you attended college. Ultimately, its up to you to include this information, I just wanted to provide some considerations. I hope this information helps. Please let me know if there are further questions! Help! I have over 22 years of clinical experience including ICU, hemodialysis, and outpatient surgery ctr (pre and pacu), and for the last 6 years (in addition to thesis service the 22 years) Ive been reading electronic charts to extract data and support level of care (Im told this is utilization review by In Beowulf:, a friend but we never called it that in work). Now Ive been laid off (it was a large comp layoff).

Im trying to thesis writing get into QA, UR, pre cert or case managment. I was told I have a solid resume but Ive gotten 3 calls in 3 months, I blew the first interview and the next 2, they said they wanted CM experience. What do I need to do to get into creole man these fields? Any suggestions? Were sorry to hear about your current situation! Its always difficult to break into an entirely new field. However, it sounds as though you have some experience to build on.

Typically, Case Management and thesis service Utilization Review require InterQual experience. Water Heaters! If you have experience with that system, then be sure to include it on your resume. Otherwise, see if you can obtain some training in it. Check with local and state agencies to see if there are any offerings for people in your situation. Thesis Writing! Also, review the specific details of each job opening and tailor your resume to include the key requirements where applicable. Check to see if there is uk a local association that you can network with like the thesis writing service, Case Management Society of America for example. Ask anyone you speak with if a Case Management Certification would improve your chances, perhaps even call the people youve interviewed with previously to see what they say.

If so, look into certification. We hope this information helps! I have been reading through some of Civil War Started? Essay this thread and was wondering if you do any resume/cover letter revisions? I can tell you put a lot of useful/productive feedback into your responses and would value your opinion if you were to review a copy of thesis writing service mine! P.S. When you mention Computer Experience in difference the article above, do you suggest adding these details under each individual job (under work experience/history) the comp. Thesis Writing Service! experience applies to or under a separate section such as the one you have listed as Comp. Experience? Thank you for all your time and effort! Thanks for reaching out! My sincerest apologies, but I do not do resume/cover letter revisions.

Thanks so much for your interest though. As for Soliloquy Essay, the computer experience, you can add it with any of the methods you described. Thesis Writing! The important thing is that its there. The resume builder on BluePipes.com lists computer experience under a separate heading. We do this as way to ensure that members recognize and Essay Interview Resources Manger record it and perspective employers and writing recruiters are able to Iago's Soliloquy easily locate the details on the members profiles and thesis service resumes. Given that EMR experience is Civil War Started? becoming such a prevalent requirement, its good to have it easily accessible on your resume. However, its also good to thesis writing service add these details under each individual job when creating your own resume.

It may not be as easy to locate, but it takes up less space, avoids redundancy, and still presents the creole man, information. We hope this information helps! I am currently an RN with 4 years solid experience in service a 16 bed transitional care unit. Now Im trying to move to a more challenging position. Iago's Essay! Prior to immigrating to thesis writing America I was a medical doctor for 9 years in ER. Would it be wise to mention that experience? maybe describing my duties and Essay about Interview Resources medical college? I work in a program that enrolls military medic and corpsman and thesis writing service gives credit for their military experience towards an intensive BSN-RN program. My question is on The In Beowulf: what should the graduates highlight on service, their resumes?

Many have extensive trauma and nursing care experience. Suggestions? Hi! I have a question / concern. I just finished my 2nd year of nursing and on a med Surg unit. I have been asked to apply to an ICU position and I need to difference between undergraduate uk update my resume. I dont know what to include. Prior to nursing, I was a surgical tech for thesis, 16+ years and active duty for 10 years.

I really enjoyed your blog and Water Essay will refer to it when updating my resume! Were glad to hear the information was useful. Thesis Service! Congratulations on Why The, being asked to apply for an ICU position. Thats a good sign! #128578; Sounds like you have a lot of great experience to include on your resume. We recommend focusing most attention on your recent experience in thesis service MedSurg as it is the most applicable to the ICU role youll be applying for. Undergraduate Uk! However, you can also include brief descriptions of your surgical tech and active duty experience as they are certainly desirable experiences. Thesis! Relate all your work history descriptions to the ICU position. To do so, find out as much as possible about the job and the unit. Analysis Essay! We hope this helps!!

Oh MY! Im sooOOO grateful to have found your blog page! I was about to do my sister a grave disservice as youve mentioned. Indeed, the thesis writing service, day of the one page crammer is passe. Especially with the value of the keyword in electronic filing. War Started? Essay! Thanks so very much; you may have saved my Siss resume from becoming fodder for the shredder. Writing Service! [[shudder]] Were glad to Analysis hear the writing service, information is useful. To be clear, 1 page resumes are still useful, particularly for job fairs or any other instance where the resume will be given directly to an individual. However, in most cases, people are attaching their resume in an Applicant Tracking System. In this case, its still good to be brief and choose your words wisely. Dont be overly verbose and Why The Civil War Started? dont keyword stuff. However, you also shouldnt be worried about writing, a 2-3 page resume if your background requires it.

Be sure to tailor the resumes wording to Iago's Essay the job posting where applicable based on your background. We hope this helps! Thank you for this article! I realized that my resume was not up to par by reading this. I had many generalized statements, which I have replaced with information on what I really did on the day to thesis writing day. I recently worked at The Benefits Heaters a hospital for 4 months and resigned due to writing it not being a good fit. It was a cardiac surgery step down unit, so it gave me experience with tele that I have not had in my 5 years as a nurse. Should I include it on my resume?

Youre welcome! Were glad to hear you found the information useful. This is a tough question. I assume youre asking because youre concerned that the short employment duration might raise some questions in the minds of potential employers. Thats a valid concern.

On the flip side, you did gain some valuable experience that would be great to Iago's Soliloquy Analysis Essay add to thesis writing your nursing resume. There is another issue to consider. Hospitals often have strict policies requiring that healthcare professionals provide them with every last bit of the healthcare professionals employment history. Omitting a previous job on Soliloquy, your employment application could be grounds for dismissal depending on how they have their clauses worded. Of course, this depends on writing, their ability to Civil Essay verify the omitted employment. This may not necessarily affect you during the candidate review process when your resume is typically the document theyre working off of.

However, if you land a job offer, then theyll eventually require an application to completed. Writing Service! If you choose to add the Soliloquy Essay, omitted job at that point, then youll have some explaining to thesis writing do. Utltimatly, the With a Human, decision is yours. If you choose to thesis writing service add the employment to your resume, then you may want to offer a brief explanation of why you left in your cover letter. Its all about the story you tell. We hope this helps. This is all great information but I do have a question. I am a recent BSN grad and licensed RN trying to land my first job.

Would you recommend including my preceptorship under clinical experience or as work experience? I have seen it both ways in examples online. Thanks, Kristin. Were glad to undergraduate uk hear this information on was useful. Youre correct, youll find preceptorship experience displayed both waysunder clinical and work experience.

To further confuse the issue, some people believe that clinical experience and thesis service work experience are one and the same while others believe they are two different things entirely. On The Sexuality! We view the writing, preceptorship as something akin to a highly advanced internship. For all intents and purposes, it is work experience. However, it doesnt constitute a traditional employment relationship. Therefore, feel free to Interview a Human Resources Manger include it under either category on your resume, but be sure to clearly indicate that its your preceptorship. On a side note, we cannot stress enough the importance of professional networking when landing your first job. Service! 70%-80% of all jobs are obtained through networking. While your resume is important, networking is the keyespecially for new grads. Creole Man! We hope this information helps. Best of luck!!

Great info I could have used that for my last job application! Do you have any tips / strategies to prepare for job interviews? I am finding these horribly stressful and hard to get my point across even when I know Id be really good at thesis writing service the job Im applying for. Thanks, Heidi! We dont have any blog posts regarding interviews yet. Thanks for the suggestion. Well explore the topic soon. Thank you! This is very informative.

Do you have any sample resume and application letter? Were glad to Soliloquy Essay hear the information is service useful, Meth! Yes, you can view our sample nursing resume which you can create for free as a member of BluePipes. You can view our recommendations on writing a nursing cover letter. We hope this helps! I LOVE this information! Thank you. Heres my question.

I have had several careers, all with different education components, dating back to the mid 1980s. How far back should I go? None relate to my current field (new nursing graduate). Its been a long time since Ive actually developed a resume and things have changedPlus, this career doesnt match those careers. Is it appropriate to ask a nursing instructor to be a reference? Are references included now-a-days?

Many, many thanks! Were glad to Iago's Soliloquy Analysis Essay hear the information is helpful!! Congratulations on thesis writing service, your recent graduation from nursing school! Yes, its a great idea to ask an instructor to be a reference. Unfortunately, your questions regarding what to include on The Benefits of Hot Water, your resume arent so cut and dry. I think its fair to thesis service say that the vast majority of Iago's Essay career consultants would say you shouldnt put references on a resume. However, if you could get a redeeming quote from a strong reference, like an thesis writing service instructor, to put in your resume summary, then it could be an eye catcher. Youre right, things have changed! We recommend reviewing our article on on The Sexuality In Beowulf:, optimizing your resume for applicant tracking systems. However, one thing is thesis writing as true today as it ever has beennetworking is the single best approach to landing a job. Iago's! We discuss the importance in service our article with recommendations for New Grad RNs.

Its tough to recommend that you leave off your past education. Id say it would be good to add any College level education as well as healthcare related education. We hope this helps!! Best of luck! Excellent information! Nursing is a second career for Essay Interview With a Human, me and writing service trying to put together a winning nursing resume has been a challenge.

You are right on the money.the resume I had for my corporate career doesnt translate well into creole man the healthcare field. I am currently looking for a new position and realized what I had on my resume wasnt going to service work. I was destined to of Hot Essay be one of those that got lost in the system. Thesis! The tips you provided have been so helpful. Creole Man! I feel confident that I am submitting a resume that will get me noticed. Thank you again for providing such valuable information. Congratulations on your new career path! Were so glad you found this information useful. We wish you the best of luck in your job search. Writing! We hope youll consider joining BluePipes.com where you can create a resume, access skills checklists for free, and build your healthcare professional network. Please excuse my shameless sales pitch #128578;

Please let us know if we can help with any questions. I have read this post with great interest. Due to a job opportunity for creole man, my husband, we moved from thesis writing service, KS to PA in 2012. Undergraduate! Even securing an interview has been daunting! I have gotten some feedback from recruiters in a large hospital in Pittsburgh, which was to writing service be sure to apply to the job requirements, which interestingly were two pages long. How is that possible? The other problem I suspect I have is the fact I have more than 20 years as a registered nurse. It seems the practice of the three major players in between undergraduate uk this area, is to lay off their experienced nurses, in service favor of hiring new grads and those with at least a year experience.

I did secure an interview which ended favorably; just short of a job offer. Iago's! The next step at this particular hospital required by the nurse recruiter, was to list ALL OF MY EXPERIENCE. I have been in nursing since 1974! LPN in thesis writing 1977 and BSN in between 1989. When she responded to my email outlining all this information, which took me more than 2 hours to complete, was we want to make certain we give you recognition for all the years you have been a nurse.

AND, that was the last I heard from thesis writing, them. I contend she realized I would have to start at about Interview a Human Resources Manger the upper end of compensation for my experience. Service! Do you know of anyone who may be advocating for the older nurse? Never thought I would use those words to describe myself I am so much more than my age. I am effective, reliable, comprehensive in my assessments, professional role model and delightful as a team member. Please give me some feedback relating to these concerns. I am ready to consult with the department of labor regarding this alleged practice to decrease costs per fte.I think its a shame! really good information here! I was updating my resume as I was reading your tips will definitely share with friends and collegues. In Beowulf: Vs. Print! Thank you so much! Hello, This was very thorough advice. Ive yet to figure how to be concise yet detailed with these tips in mind.

I have almost 2 years experience in In-Patient Psychiatry and SNF just because those were the thesis service, two jobs I landed. However, I always wanted to do LD or NICU. I did my preceptorship in 2011 in LD. How can I use my experience and make it appealing for LD again? Were glad to hear that you found the information useful! You can view the BluePipes sample resume to get an Civil Essay idea for formatting yours. Please bear in mind that our site generates a PDF document and you could get more compact results with a word processor like Microsoft Word.

Also, you might be interested in reading our post on resume length. You can find creative ways to translate your Psych and SNF experience to writing NICU and L#038;D. However, you most likely wont be able to address the and postgraduate uk, unique technical skills required for these units. You could look for thesis writing service, volunteer opportunities working with pregnant women and newborns in your community. While its always tough to Soliloquy find time to volunteer, youll be doing a great service for your community and getting some relevant experience to add to your resume. Thank you for thesis writing, this very interesting article.

I have been an RN for 9 years; 4 years at the bedside and 5 years in a hospital-based surgical practice doing outpatient, telehealth and some inpatient care. I would like to transition back to The Benefits the bedside but feel that my lack of direct patient care over the last 5 years may be hamstringing me. How can I turn this perceived negative into a positive and at least get through the front door of the service, interview process? Youre welcome, we hope you found the article useful. Of Hot Water Heaters Essay! Yours is a legitimate challenge that many nurses share. With respect to your resume, you should focus on demonstrating how your recent experience translates to bedside nursing. While were not familiar with your specific scenario, were certain that there are aspects of your experience with outpatient, telehealth and inpatient care that translate to bedside nursing. Carefully review the job duties and qualifications of bedside nurses, and writing not just those found in the job descriptions of online job postings, to get ideas for framing your recent experience in creole man a way that applies to bedside nursing. Next, focus on writing an excellent nursing cover letter in which you present yourself as a solution to the problems facing the employer in question. Of course, youre still going to thesis writing service have some difficulties ranking highly in the applicant tracking system due to the lack of creole man recent bedside experience.

Therefore, networking is writing service a must. Nearly 40% of all new-hires are the result of employee referral. With 9 years of experience, youre bound to have some connections and now is the time to leverage them. We realize this type of job searching can feel unorthodox, but its success rate is between too good to thesis writing service neglect it. Use professional networking sites like BluePipes and LinkedIn to enhance your networking efforts. Finally, weve spoken to many nurses who tout the benefits of refresher courses for bedside nursing. Iago's Analysis! While we arent familiar with such courses, the fact that many nurses recommend them is an indication that they should be further researched as a potential tool when facing your challenge.

We hope this information helpsand Good Luck!! i have a question regarding applying to nursing positions. Thesis! I am a new grad RN and have several nursing job applications that have been under review for over a month. Last week I became certified in ACLS and was wondering how to go about informing the hospitals that I am waiting to hear back from Soliloquy Analysis Essay, that I am newly certified in this skill, since it is not on thesis, my resume that they have on file (that I originally sent in). Congratulations on your recent achievements! This is a great question. You may be able to log in to the hospitals applicant tracking systems (the online system you most likely used to Why The Civil Essay apply) and update your resume and/or profile. Either way, you may also want to writing give them a call, ask for the staffing office and pose this question to the representative you speak with. We find that hospitals are quite responsive to candidate inquiries relative to on The Sexuality In Beowulf: other employers.

We hope these recommendations help, and wed love to hear back about what actions you take and how they work out. Good luck! I completely disagree that availability should be included on the resume. When a nurse applies for thesis writing service, a position it is creole man usually clear what hours that he/she is applying to work. Thesis Writing Service! I actually think its a bit juvenile to The Benefits Water Heaters Essay put availability on your resume. What I do think that many nurses omit is a well-crafted cover letter to accompany their resume. The additional touch of a thoughtful and appropriate cover-letter can help give you an edge over other applicants who omit this step. Writing! I found some good details on cover letters for nurses here: http://bit.ly/1dDy8If . Thanks for creole man, the feedback, Brittney. Thesis Writing Service! Including availability on a nursing resume is important for many reasons.

First, the job applied for isnt the only job available, and between and postgraduate most jobs are never advertised so candidates will never really know about all of the available jobs with a particular employer. Second, job specs dont always display every last pertinent detail. With respect to availability, this can mean that a job advertised for day shifts might in-fact be for writing, mid shifts, or pm shifts. This happens frequently when the job board technology limits the employers ability to accurately display the With Manger, shifts. Third, job specs often change on the back-end and are never updated on the front-end. So a job thats advertised for days may have changed to nights without being updated on the job board. Fourth, due to the proliferation of applicant tracking systems, resumes are added to a searchable database these days. Thesis Writing! So resumes are searchable for difference between undergraduate, future job openings. Finally, recruiters will almost always contact the candidate with the most attributes in common with any given job description. So in all of the scenarios described above, a recruiter will be more prone to contact the candidate with matching availability assuming all else is writing equal. Finally, its true that nursing resumes should always be tailored for a specific job description when a specific job is being applied for.

However, a plurality of jobs are filled via networking and referrals. In such cases, its rare that a specific job is Essay Sexuality In Beowulf: Film vs. Print being applied for. Instead, a candidate passes their resume along to thesis writing a contact who has connections with the between undergraduate uk, employer in thesis service question. The resume is reviewed for potential matches with available jobs and the candidate is contacted. Again, availability is one detail that recruiters and hiring managers are looking for. Lastly, we agree that cover letters are important and Essay about Resources Manger related to this topic. However, they necessitate their own discussion and we plan on addressing this in a future blog post. This is great information! As a new graduate RN with no prior experience in the healthcare field what would you recommend? I have over 4 years in retail but Im not sure if this would make much of a difference for recruiters.

Any help is greatly appreciated, thanks! Hi there! Wow this information is great! Although Im still an thesis service undergraduate, I find this site very helpful for tips to creole man keep in mind when applying for a job! I just had a concern that may affect my decision within the Nursing field For a while, I was thinking of minoring in something in addition to Nursing that I can apply to the work field.

I know that computer knowledge is a great booster for a resume, but I wanted to thesis service know if there were any other skills hospitals are looking for Essay on The Sexuality, in their RNs? Thank you again for thesis, the detailed explanations! Thanks, Michele! Were glad you found the Iago's Soliloquy, information useful! Yes, computer knowledge is a great booster for the resume.

You can look into Health Care Informatics as an service option for of Hot Water Heaters Essay, a minor or additional coursework. You may also consider Healthcare Management or Administration, Nutritional Sciences, Biology, Sign Language, or Spanish. In fact, Spanish is writing a huge selling point these days. As a side note: make the absolute most of your preceptorship. Be at your best and use the opportunity to network with everyone you can. We hope this information helps!! Please let us know if you have any other questions. This is great information! Thank you! I am wondering though, as a RN with 1 year of experience in the CVICU, trying to Analysis Essay move, with most places wanting more experience than that for thesis service, hire, how should I market myself?

I have done all of these critical care elements, but just not a ton of The Benefits Heaters it. Thesis Service! Before nursing school, I was a critical care telemetry tech for Essay With Manger, 3 years and a hospital pharmacy tech of service 3 years as well as a SNE(student nurse extern) during school. Because it is not nursing, but healthcare related, should I include it in my resume? It seems juvenile but pertinent to add some experience. Thanks! Were happy to hear you found the information helpful, Dashia! You pose an excellent question regarding the inclusion of non-RN healthcare experience on your resume.

There isnt a steadfast rule, unfortunately, and youre going to find that some people will say yes and some people will say no. I fall in the yes camp and believe you should include all healthcare related experience on your resume for about Interview a Human Resources, several reasons. Thesis Writing Service! First, it demonstrates progression within the general field of healthcare. Second, it conveys additional experience within the field that other candidates may not have. Third, in Essay on The Sexuality vs. Print many cases (and certainly in your case) the service, experience is related to Why The the jobs youre applying for. Writing Service! Fourth, it may help you stand out from other candidates with otherwise similar experience. Fifth, unbeknownst to Essay vs. Print many candidates, many hospitals require your last 7 to 10 years of work history regardless of thesis writing service whether or not it was healthcare related. While this requirement doesnt factor into difference between and postgraduate their candidate selection process, they will certainly collect the information at some point during the writing service, hiring process. Finally, it may help your resume rank higher in applicant tracking systems. Iago's Essay! I hope this information helps! This information is thesis writing very helpfull.

Hi. I obtained an MD degree overseas before I even started getting licensed here as RN. I was told it would not help or matter anymore if I include that in my resume. What would you suggest? Hey Liza, I hope all is well. I recommend including your overseas education. I agree that it most likely will not influence the initial resume screening process. However, it could have influence later in the process. You may not want to make it a significant part of your resume, but I think including it is Iago's Soliloquy Analysis Essay better than not. Thesis! It certainly enhances your personal story.

Do you have an example resume? Hey Michaele, My apologies for the delay. Weve been busy transferring our blog to this new domain. We do not have an example nurse resume that were able to publish to on The Sexuality Of Women In Beowulf: the blog at this time. Writing Service! However, we will work on getting one up soon. Also, I recommend becoming a member on Why The Essay, BluePipes where your professional profile will allow you to record the top 10 details recommended for nursing resumes in thesis writing service this article. You can then print your BluePipes profile as a resume. Thanks so much!! This clarifies a lot for me. I am a new LPN attempting to start my career in a new state and have read a lot of advice on Nurses.com that is Essay on The Film vs. Print wrong.

Ill take the advice of a recruiter over general opinions anytime. Most on the other sites were saying NEVER to include your license # details until you actually get a job offer. Then I just applied to writing one that specifically asked that I provide that info as well as any certifications on my resume in order to be considered. I was hesitant since all the Essay on The Of Women Film vs. Print, threads warn of protecting it at writing service all cost due to identity theft? Im glad you clarified this and some other points. I do have one question that is not addressed in your article. If you are continuing your education to receive your BSN or MSN for example would mentioning that on The Benefits, your resume make a candidate more desirable to thesis writing service potential employers or would you leave that off? Hey Aimee, Im glad to Essay on The Sexuality Of Women Film vs. Print hear that youve found the writing, information useful. Great question!! I think its a great idea to about Interview With a Human Manger add your education in progress to your resume.

I recommend including an estimated completion date and perhaps even the number of units completed to date. There are several reasons adding your education in progress could be beneficial. First, it could result in writing service a higher ranking by the Applicant Tracking System. Second, it shows that youre interested in advancing your knowledge and your career. Third, it demonstrates your own personal motivation. As for the identity theft claims: Your license number is a matter of public record already.

Anybody can go to creole man your state board of nursing and search by thesis service, name to Soliloquy verify your state license, which includes the license number. So, even letting someone know your name, that youre a registered nurse, and the state youre licensed in provides the means to easily obtain your license number. Providing it on writing, your resume simply allows the human resources rep to Essay Of Women Film vs. Print skip this step. Which increases the chance that your resume will be passed up the chain. And remember, youre attaching your resume in writing service secure Applicant Tracking Systems, many of which also ask for your Social Security Number and Date of Why The Civil War Started? Birth (although some states prohibit employers from thesis service, asking for SSN on job applications). I recently completed my ADN, passed the difference and postgraduate, NCLEX, and now I am working on my resume. I already have a BA and MSW, which I will include on my resume, but I am wondering how I can state that I intend to pursue a BSN, although have not yet applied and writing service dont have credits towards the degree.

Thanks for your advice. Congratulations on your recent achievements! You pose a great question regarding RN resumes, one for which there is no steadfast answer. My belief is that it wouldnt be appropriate to include the information in the Education section of your resume because you havent yet been accepted to Civil a BSN program. Thesis Writing! However, you could certainly add it there once accepted to a program. Until then, you could make brief mention of this information in your nursing resume summary. We hope you find this response useful. Difference Between Undergraduate! Please feel free to post any follow-up or additional questions you may have. Thesis Writing! Good luck!! I have an additional question regarding previous education that I havent seen asked by anyone. Im an older new grad.

I recently had someone look at creole man one of my resumes and give me her input. She deleted all but my nursing education (Associates Degree). In some ways I think its not a bad idea since the previous education dates me if I include the years I attended (which I dont but suppose that omission might make HR wonder). On the other hand, I attended highly regarded universities for undergrad and grad studies, not to mention it shows I have a Bachelors even though its not in the sciences. Writing Service! It seems like the RN to BSN courses are more human and social science courses, rather than science courses, however, and about With a Human Resources that is the majority of my BA.

OK Thats some background info, now Ill get to the point #128578; I know that resumes should cover only the last 10 years of employment history. Do you think this rule also applies to education? Should I leave it to discuss in an interview (as recommended by the person who revised my resume)? Im feeling very conflicted about this. The new resume doesnt feel like me, my degrees can be related to writing the psycho-social aspect of nursing, and when you fill in The Benefits of Hot Water the online application portion they ask for all education (although you usually have to add on education). I would greatly appreciate your insight and recommendation. My sincerest apologies for the delay. Congratulations on your recent graduation! This is a tough situation. Both sides of the writing, argument have merit. Moreover, there really arent any steadfast rules about resumesonly staunch opinions.

To me, your nursing resume and cover letter are all about telling your story in a way that convinces the reader youre the right person for creole man, the job while providing them with the details they need. Personally, Id keep the education details on the resume, and use a brief portion of the thesis, cover letter to tie that education into the story about creole man, you being the thesis, best candidate for the job in question. Given the Interview a Human Resources, information youve provided about your previous education, it sounds as though you have a great opportunity to do just that. Again, there is no right or wrong answer here. Writing! You may even try it both ways. Some readers will appreciate it one way and other will appreciate it another.

However, Im of the belief that all education is valuable. I hope this information helps. Wed love to hear back about how it all works out!!

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8 Cover Letter Tips and a Sample Cover Letter For Your Law Firm Internship. The cover letter is a small email you write while applying to a law firm. It is thesis service really your pitch which says #8220;Take me as an intern#8221; Draft it carefully and do note the Water, following points. 1. The body of the email should itself contain the thesis, cover letter . Don#8217;t attach a 300 word cover letter because it won#8217;t be opened in ten cases out of nine. Ten out of nine?

It might seem an exaggeration or a misprint but it is none. Instead of sending your cover letter as an attachment, write it in the body of the email itself. 2. Subject of the email which is the subject of your cover letter should be detailed and educated, a bit like this: Internship Application: 1 st May- 30 th June: Mumbai Office. These are the on The Film, three things (subject + duration + location) should make the subject of your internship cover letter; nothing more and nothing less. 3. Focus and customise. Writing Service? Don#8217;t send an Of Women Film vs. Print, email to dozens of law firms at one go. Identify 5-6 law firms which interest you and send applications tailor-made for them. For example if you are applying to an IP boutique law firm, include a section in your CV (right at the top, mind you) which reads #8216;Achievements in the field of IP#8217;. Also in your cover letter mention these achievements precisely. 4. Art of the writing service, start.

Start with naming the person you want this to read; the recruitment head, the managing partner or the internship coordinator. Do your research. Assuming the name of the person is Ram Kumar and the email id for internships is [emailprotected] and you start with #8220;Respected Mr. Ram Kumar#8221;? you catch his attention. If you begin with Respected Sir/Madam you don#8217;t get anyones attention. 5. Write English . Essay Interview With A Human? Please don#8217;t show of your legal writing skills here. Write simple, plain, correct, readable English. Reserve legalese for later, when you actually get the internship. Do a spelling and grammar check on Word on writing service your cover letter. 6. Attach your CV. If you are not careful with this little thing (of attaching files), the law firm will be unwilling to hand you bigger things like internship.

7. Ask. Ask if you should be sending your writing samples or if the Film vs. Print, firm will want to take a telephonic interview. Leave your contact number in thesis service, the footer. Difference Between Undergraduate? 8. A little quote. Emails can have a quote at the end. If it relates to law, is a smart one and is something you identify with, you can include it in every email you send including the thesis, internship application. This might be a little controversial but it can tell the recruiter a lot about Iago's Essay, you. It adds a human touch and might bring a smile on the recruiters face. A smiling man/woman is a person more likely to give you the internship.

So choose the thesis, quote carefully. Here is a sample cover letter for a law firm internship: Dear Mr. Undergraduate And Postgraduate? Ram Kumar. This section is: Art of start: Who you are, what you do and where are you going. I am Rahul Sharma, a 4 th year law student from Gujarat National Law University (GNLU), Gandhinagar and thesis writing am very keen to intern at ABC Law Offices (Delhi) from 1 st May- 30 th June.

This is called: Why Me. The Best Things I Have Done. Why Your Firm Alone is the Perfect Professional Habitat For Me. I have previously interned at XYZ#8217;s Delhi office where I was a part of the Capital Markets team. During my internship under Mr. Mukul Romani, senior counsel, Supreme Court of India I learnt the basics of legal drafting under his tutelage. I have also completed the Iago's, Mergers and Acquisitions course offered by the Bombay Stock Exchange. Moreover, I was a part of the ANLU team (oralist) which won the NUKS corporate law moot competition in writing, 2010. I am sure interning at a prestigious law firm like SRGR Law Offices will help me further improve upon my skills.

Given my prior experience and interest in on The Of Women In Beowulf:, corporate law I am sure that Ill be able to contribute immensely towards your practice. I have learnt from LegallyIndia.com about writing service, your firm#8217;s growth plans and it will be exciting to be a part of a young and of Hot Heaters growing team of lawyers. The Section: I am humble, eager and good. I am attaching my CV for your kind perusal and will be grateful if I am given an opportunity to intern at your firm. Thesis? Please let me know if you require anything else as a part of the internship application. Thank you for your time and consideration. 4 th year student, BA.LLB (Hons) course. Contact: 999999999 (M) P.S.

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